CaltechAUTHORS
  A Caltech Library Service

Molecular dissection of Penelope transposable element regulatory machinery

Schostak, Nataliya and Pyatkov, Konstantin and Zelentsova, Elena and Arkhipova, Irina and Shagin, Dmitrii and Shagina, Irina and Mudrik, Elena and Blintsov, Anatolii and Clark, Ivan and Finnegan, David J. and Evgen'ev, Michael (2008) Molecular dissection of Penelope transposable element regulatory machinery. Nucleic Acids Research, 36 (8). pp. 2522-2529. ISSN 0305-1048. https://resolver.caltech.edu/CaltechAUTHORS:SCHOnar08

[img]
Preview
PDF (Advance Access)
See Usage Policy.

1483Kb
[img]
Preview
PDF
See Usage Policy.

1175Kb
[img]
Preview
PDF (Supplementary Data 1)
See Usage Policy.

71Kb
[img]
Preview
PDF (Supplementary Data 2)
See Usage Policy.

91Kb
[img]
Preview
PDF (Supplementary Data 3)
See Usage Policy.

13Kb
[img] Other (Supplementary Data 1 (.doc))
See Usage Policy.

91Kb
[img] Other (Supplementary Data 2 (.doc))
See Usage Policy.

54Kb
[img] Other (Supplementary Data 3 (.doc))
See Usage Policy.

29Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:SCHOnar08

Abstract

Penelope-like elements (PLEs) represent a new class of retroelements identified in more than 80 species belonging to at least 10 animal phyla. Penelope isolated from Drosophila virilis is the only known transpositionally active representative of this class. Although the size and structure of the Penelope major transcript has been previously described in both D. virilis and D. melanogaster transgenic strains, the architecture of the Penelope regulatory region remains unknown. In order to determine the localization of presumptive Penelope promoter and enhancer-like elements, segments of the putative Penelope regulatory region were linked to a CAT reporter gene and introduced into D. melanogaster by P-element-mediated transformation. The results obtained using ELISA to measure CAT expression levels and RNA studies, including RT–PCR, suggest that the active Penelope transposon contains an internal promoter similar to the TATA-less promoters of LINEs. The results also suggest that some of the Penelope regulatory sequences control the preferential expression in the ovaries of the adult flies by enhancing expression in the ovary and reducing expression in the carcass. The possible significance of the intron within Penelope for the function and evolution of PLEs, and the effect of Penelope insertions on adjacent genes, are discussed.


Item Type:Article
Additional Information:© 2008 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Received July 31, 2007. Revised December 15, 2007. Accepted December 18, 2007. Nucleic Acids Research Advance Access published online on March 4, 2008, Nucleic Acids Research, doi:10.1093/nar/gkm1166 We are grateful to Dr Boris Andrianov (Institute of General Genetics RAS, Moscow) for performing transient expression experiments exploring D. virilis cell culture. We thank Dr Olga Zatsepina for critically reading the manuscript and fruitful discussion of the results. This work was supported by grants from Russian Academy of Sciences (Cell and Molecular Biology to M.E.), and Welcome Trust Grant (075698) to M.E and D.J.F. Funding to pay the Open Access publication charges for this article was provided by The Wellcome Trust. Conflict of interest statement. None declared. The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors. Supplementary Data are available at NAR Online.
Issue or Number:8
Record Number:CaltechAUTHORS:SCHOnar08
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:SCHOnar08
Alternative URL:http://dx.doi.org/10.1093/nar/gkm1166
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10047
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:08 Apr 2008
Last Modified:03 Oct 2019 00:06

Repository Staff Only: item control page