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Tissue clearing and its applications in neuroscience

Ueda, Hiroki R. and Ertürk, Ali and Chung, Kwanghun and Gradinaru, Viviana and Chédotal, Alain and Tomancak, Pavel and Keller, Philipp J. (2020) Tissue clearing and its applications in neuroscience. Nature Reviews. Neuroscience, 21 (2). pp. 61-79. ISSN 1471-003X. https://resolver.caltech.edu/CaltechAUTHORS:20200106-082556970

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Abstract

State-of-the-art tissue-clearing methods provide subcellular-level optical access to intact tissues from individual organs and even to some entire mammals. When combined with light-sheet microscopy and automated approaches to image analysis, existing tissue-clearing methods can speed up and may reduce the cost of conventional histology by several orders of magnitude. In addition, tissue-clearing chemistry allows whole-organ antibody labelling, which can be applied even to thick human tissues. By combining the most powerful labelling, clearing, imaging and data-analysis tools, scientists are extracting structural and functional cellular and subcellular information on complex mammalian bodies and large human specimens at an accelerated pace. The rapid generation of terabyte-scale imaging data furthermore creates a high demand for efficient computational approaches that tackle challenges in large-scale data analysis and management. In this Review, we discuss how tissue-clearing methods could provide an unbiased, system-level view of mammalian bodies and human specimens and discuss future opportunities for the use of these methods in human neuroscience.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1038/s41583-019-0250-1DOIArticle
https://rdcu.be/b0R5FPublisherFree ReadCube access
https://doi.org/10.1038/s41583-020-0291-5DOIPublisher Correction
https://rdcu.be/b21swPublisherFree ReadCube access - Publisher Correction
ORCID:
AuthorORCID
Ueda, Hiroki R.0000-0001-8342-9176
Ertürk, Ali0000-0001-5163-5100
Chung, Kwanghun0000-0002-8167-3340
Gradinaru, Viviana0000-0001-5868-348X
Chédotal, Alain0000-0001-7577-3794
Keller, Philipp J.0000-0003-2896-4920
Additional Information:© 2020 Springer Nature Limited. Accepted 18 November 2019; Published 02 January 2020. The authors thank E. A. Susaki for help in compiling Table 1 and Supplementary Table 1 for current tissue-clearing protocols and reagents, K. Matsumoto and Y. Shinohara for drawing the chemical structures in the supplementary information, T. Mano for contributing to the CUBIC figure, R. Cai and C. Pan for contributing to the uDISCO figure, S. R. Kumar, G. M. Coughlin, R. Challis and C. Challis for contributing to the viral-assisted spectral tracing figure and Y.-G. Park, C. H. Sohn, T. Ku, V. Lilascharoen and B. K. Lim for contributing to the SHIELD figure. The authors also gratefully acknowledge grant support from Brain/MINDS, the Basic Science and Platform Technology Program for Innovative Biological Medicine (AMED/MEXT), the Japan Society for the Promotion of Science (Grant-in-Aid for Scientific Research (S)) and the Human Frontier Science Program Research Grant Program (HFSP RGP0019/2018) (H.R.U.), the Munich Cluster for Systems Neurology (SyNergy), the Fritz Thyssen Stiftung and the Deutsche Forschungsgemeinschaft (A.E.), the David and Lucile Packard Foundation (Packard Fellowship), the McKnight Foundation, the US National Institutes of Health (NIH) (1-DP2-ES027992; U01MH117072), the NCSOFT Cultural Foundation and the Koreaan Institute for Basic Science (IBS-R026-D1) (K.C.), the NIH BRAIN Initiative, the NIH Office of the Director and the US National Science Foundation (NeuroNex) (V.G.), LABEX LIFESENSES (reference ANR-10-LABX-65) managed by the French Agence National de la Recherche within the Investissements d’Avenir programme under reference ANR-11-IDEX-0004-02 (A.C.), the European Regional Development Fund in the framework of the Czech IT4Innovations National Supercomputing Center path to exascale project, project number CZ.02.1.01/0.0/0.0/16_013/0001791, within the Czech Research, Development and Education Operational Programme (P.T.) and the Howard Hughes Medical Institute (P.J.K.). Author Contributions: H.R.U., A.E., K.C., V.G., A.C. and P.J.K. researched data for the article. All the authors contributed to substantial discussion of its content, wrote the article and reviewed and edited the manuscript before submission. Competing interests: H.R.U. is a co-inventor on a patent applications covering the CUBIC reagents (PCT/JP2014/070618 (pending), patent applicant is RIKEN, other co-inventors are E. A. Susaki and K. Tainaka; PCT/JP2017/016410 (pending), patent applicant is RIKEN, other co-inventors are K. Tainaka and T. Murakami) and a co-founder of CUBICStars Inc. A.E. is the applicant and the inventor on a patent application for technologies relating to vDISCO clearing (PCT/EP2018/063098 (pending)). K.C. is the inventor or a co-inventor on patents and patent applications for CLARITY (PCT/US2013/031066 (active), patent applicant is Stanford University, co-inventor is K. A. Deisseroth), stochastic electrotransport (PCT/US2015/024297 (active), patent applicant is MIT), SHIELD (PCT/US2016/064538 (pending), applicant is Massachusetts Institute of Technology (MIT), other co-inventors are E. Murray and J. H. Cho), SWITCH (PCT/US2016/064538 (pending), applicant is MIT, other co-inventors are E. Murray and J. H. Cho) and MAP (PCT/US2017/030285 (pending), applicant is MIT, other co-inventors are T. Ku, J. M. Swaney and J. Y. Park) and a co-founder of LifeCanvas Technologies. V.G. is a co-inventor on patent applications covering PACT and PARS (PCT/US2014/048985 (active), applicant is California Institute of Technology, other co-inventors are V. Gradinaru and B. Yang) and adeno-associated virus (US14/485,024 (active), applicant is California Institute of Technology, other co-inventors are B. E. Deverman, P. H. Patterson and V. Gradinaru) technologies. P.J.K. is an inventor or co-inventor on patents and patent applications covering multiview imaging (US14/049,470 (active), applicant is Howard Hughes Medical Institute) and adaptive light-sheet microscopy (PCT/US2017/038970 (pending), applicant is Howard Hughes Medical Institute, other co-inventors are R. K. Chhetri and L. A. Royer). P.T. and A.C. declare no competing interests.
Errata:In this Review Article, the accept date was inadvertently omitted. It should be 18 November 2019. This error has been corrected online.
Funders:
Funding AgencyGrant Number
Brain Mapping by Integrated Neurotechnologies for Disease StudiesUNSPECIFIED
Basic Science and Platform Technology Program for Innovative Biological MedicineUNSPECIFIED
Ministry of Education, Culture, Sports, Science and Technology (MEXT)UNSPECIFIED
Japan Society for the Promotion of Science (JSPS)UNSPECIFIED
Human Frontier Science ProgramRGP0019/2018
Munich Cluster for Systems Neurology (SyNergy)UNSPECIFIED
Fritz Thyssen StiftungUNSPECIFIED
Deutsche Forschungsgemeinschaft (DFG)UNSPECIFIED
David and Lucile Packard FoundationUNSPECIFIED
McKnight FoundationUNSPECIFIED
NIH1-DP2-ES027992
NIHU01MH117072
NCSOFT Cultural FoundationUNSPECIFIED
Korean Institute for Basic ScienceIBS-R026-D1
NSFUNSPECIFIED
Agence Nationale pour la Recherche (ANR)ANR-10-LABX-65
Agence Nationale pour la Recherche (ANR)ANR-11-IDEX-0004-02
European Regional Development FundUNSPECIFIED
Czech Research, Development and Education Operational ProgrammeCZ.02.1.01/0.0/0.0/16_013/0001791
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Subject Keywords:Immunological techniques; Light-sheet microscopy; Neuroscience
Issue or Number:2
Record Number:CaltechAUTHORS:20200106-082556970
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200106-082556970
Official Citation:Ueda, H.R., Ertürk, A., Chung, K. et al. Tissue clearing and its applications in neuroscience. Nat Rev Neurosci 21, 61–79 (2020). https://doi.org/10.1038/s41583-019-0250-1
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:100523
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:07 Jan 2020 19:42
Last Modified:16 Mar 2020 22:17

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