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Protein phosphatase 1 regulation by inhibitors and targeting subunits

Watanabe, Takuo and Huang, Hsien-Bin and Horiuchi, Atsuko and Silva, Edgar F. and Hsieh-Wilson, Linda and Allen, Patrick B. and Shenolikar, Shirish and Greengard, Paul and Nairn, Angus C. (2001) Protein phosphatase 1 regulation by inhibitors and targeting subunits. Proceedings of the National Academy of Sciences of the United States of America, 98 (6). pp. 3080-3085. ISSN 0027-8424. PMCID PMC30610.

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Regulation of protein phosphatase 1 (PP1) by protein inhibitors and targeting subunits has been previously studied through the use of recombinant protein expressed in Escherichia coli. This preparation is limited by several key differences in its properties compared with native PP1. In the present study, we have analyzed recombinant PP1 expressed in Sf9 insect cells using baculovirus. Sf9 PP1 exhibited properties identical to those of native PP1, with respect to regulation by metals, inhibitor proteins, and targeting subunits, and failure to dephosphorylate a phosphotyrosine-containing substrate or phospho-DARPP-32 (Dopamine and cAMP-regulated phosphoprotein, M-r 32,000). Mutations at Y272 in the beta 12/beta 13 loop resulted in a loss of activity and reduced the sensitivity to thiophospho-DARPP-32 and inhibitor-2. Mutations of Y272 also increased the relative activity toward a phosphotyrosine-containing substrate or phospho-DARPP-32. Mutation of acidic groove residues caused no change in sensitivity to thiophospho-DARPP-32 or inhibitor-2, but one mutant (E252A:D253A:E256R) exhibited an increased K-m for phosphorylase a. Several PP1/PP2A chimeras were prepared in which C-terminal sequences of PP2A were substituted into PP1. Replacement of residues 274-330 of PP1 with the corresponding region of PP2A resulted in a large loss of sensitivity to thiophospho-DARPP-32 and inhibitor-2, and also resulted in a loss of interaction with the targeting subunits, spinophilin and PP1 nuclear targeting subunit (PNUTS). More limited alterations in residues in beta 12, beta 13, and beta 14 strands highlighted a key role for M290 and C291 in the interaction of PP1 with thiophospho-DARPP-32, but not inhibitor-2.

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Hsieh-Wilson, Linda0000-0001-5661-1714
Additional Information:© 2001 by the National Academy of Sciences. Contributed by Paul Greengard, January 3, 2001. This work was supported by U.S. Public Service Grants MH40899 (to A.C.N. and P.G.) and DK52054 (to S.S.), and by Grant NSC 90-2316-8320-002 from the National Science Council of Taiwan (to H.-B.H.). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
National Science Council (Taipei)NSC 90-2316-8320-002
Subject Keywords:catalytic subunit, binding, muscle, conformation, spinophilin, chaperone, DARPP-32, complex, domains, enzyme
Issue or Number:6
PubMed Central ID:PMC30610
Record Number:CaltechAUTHORS:WAYpnas01
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1007
Deposited By: Tony Diaz
Deposited On:29 Nov 2005
Last Modified:12 Dec 2019 17:06

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