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Engineering new catalytic activities in enzymes

Chen, Kai and Arnold, Frances H. (2020) Engineering new catalytic activities in enzymes. Nature Catalysis, 3 (3). pp. 203-213. ISSN 2520-1158. doi:10.1038/s41929-019-0385-5.

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The efficiency, selectivity and sustainability benefits offered by enzymes are enticing chemists to consider biocatalytic transformations to complement or even supplant more traditional synthetic routes. Increasing demands for efficient and versatile synthetic methods, combined with powerful new discovery and engineering tools, has prompted innovations in biocatalysis, especially the development of new enzymes for precise transformations or ‘molecular editing’. As a result, the past decade has witnessed an impressive expansion of the catalytic repertoire of enzymes to include new and useful transformations not known (or relevant) in the biological world. In this Review we illustrate various ways in which researchers have approached using the catalytic machineries of enzymes for new-to-nature transformations. These efforts have identified genetically encoded catalysts that can be tuned and diversified by engineering the protein sequence, particularly by directed evolution. Discovery and improvement of these new enzyme activities is opening a floodgate that connects the chemistry of the biological world to that invented by humans over the past 100 years.

Item Type:Article
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URLURL TypeDescription ReadCube access
Chen, Kai0000-0002-3325-3536
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2020 Springer Nature Limited. Received 25 August 2019; Accepted 21 October 2019; Published 20 January 2020. We thank D. J. Wackelin and Y. Yang (Caltech) for helpful discussions and comments on the manuscript. This work was supported by NSF Division of Molecular and Cellular Biosciences grant no. MCB-1513007, the US Army Research Office Institute for Collaborative Biotechnologies (cooperative agreement no. W911NF-19-2-0026) and the US Army Research Office Institute for Collaborative Biotechnologies (contract no. W911NF-19-D-0001). K.C. thanks the Resnick Sustainability Institute at Caltech for fellowship support. The content of this paper does not necessarily reflect the position or the policy of the funding agencies, and no official endorsement should be inferred. Author Contributions: All authors participated in designing and writing the manuscript. The authors declare no competing interests.
Group:Resnick Sustainability Institute
Funding AgencyGrant Number
Army Research Office (ARO)W911NF-19-2-0026
Army Research Office (ARO)W911NF-19-D-0001
Resnick Sustainability InstituteUNSPECIFIED
Subject Keywords:Biocatalysis; Biosynthesis; Catalysis; Enzymes
Issue or Number:3
Record Number:CaltechAUTHORS:20200121-114421759
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Official Citation:Chen, K., Arnold, F.H. Engineering new catalytic activities in enzymes. Nat Catal 3, 203–213 (2020).
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:100816
Deposited By: Tony Diaz
Deposited On:21 Jan 2020 19:49
Last Modified:16 Nov 2021 17:57

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