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In situ correction of liquid meniscus in cell culture imaging system based on parallel Fourier ptychographic microscopy (96 Eyes)

Pan, An and Chan, Antony C. S. and Yao, Baoli and Yang, Changhuei (2019) In situ correction of liquid meniscus in cell culture imaging system based on parallel Fourier ptychographic microscopy (96 Eyes). . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20200203-082313476

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Abstract

We collaborated with Amgen and spent five years in designing and fabricating next generation multi-well plate imagers based on Fourier ptychographic microscopy (FPM). A 6-well imager (Emsight) and a low-cost parallel microscopic system (96 Eyes) based on parallel FPM were reported in our previous work. However, the effect of liquid meniscus on the image quality is much stronger than anticipated, introducing obvious wavevector misalignment and additional image aberration. To this end, an adaptive wavevector correction (AWC-FPM) algorithm and a pupil recovery improvement strategy are presented to solve these challenges in situ. In addition, dual-channel fluorescence excitation is added to obtain structural information for microbiologists. Experiments are demonstrated to verify their performances. The accuracy of angular resolution with our algorithm is within 0.003 rad. Our algorithms would make the FPM algorithm more robust and practical and can be extended to other FPM-based applications to overcome similar challenges.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
http://arxiv.org/abs/1912.00804arXivDiscussion Paper
ORCID:
AuthorORCID
Chan, Antony C. S.0000-0002-5104-261X
Yang, Changhuei0000-0001-8791-0354
Additional Information:We would like to thank Albert Chung and Daniel Martin (California Institute of Technology, USA) for helpful discussions. The authors have no relevant financial interests in this article and no potential conflicts of interest to disclose. Funding: Caltech Agency Award (AMGEN.96EYES).
Funders:
Funding AgencyGrant Number
CaltechAMGEN.96EYES
Record Number:CaltechAUTHORS:20200203-082313476
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200203-082313476
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:101048
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:03 Feb 2020 16:48
Last Modified:03 Feb 2020 16:48

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