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Stochastic Gene Expression in Single Gene Oscillator Variants

Swaminathan, Anandh and Gomez, Marcella M. and Shis, David L. and Bennett, Matthew R. and Murray, Richard M. (2016) Stochastic Gene Expression in Single Gene Oscillator Variants. . (Unpublished)

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It is infeasible to understand all dynamics in cell, but we can aim to understand the impact of design choices under our control. Here we consider a single gene oscillator as a case study to understand the influence of DNA copy number and repressor choice on the resulting dynamics. We first switch the repressor in the oscillator from the originally published lacI to treRL, a chimeric repressor with a lacI DNA binding domain that is inducible by trehalose. This slightly modified system produces faster and more regular oscillations than the original lacI oscillator. We then compare the treRL oscillator at three different DNA copy numbers. The period and amplitude of oscillations increases as the copy number is decreased. We cannot explain the change in period with differential equation models without changing delays or degradation rates. The correlation and phase coherence between daughter cells after cell division also tend to fall off faster for the lower copy oscillator variants. These results suggest that lower copy number variants of our single gene oscillator produce more synchronized oscillations.

Item Type:Report or Paper (Discussion Paper)
Swaminathan, Anandh0000-0001-9935-6530
Murray, Richard M.0000-0002-5785-7481
Additional Information:A. Swaminathan acknowledges the NSF GRFP for funding. A. Swaminathan and R.M. Murray acknowledge AFOSR grant FA9550-14-1-0060 for funding. The authors are grateful to E. Yeung, V. Hsiao, and A. Thubagere for experimental advice and discussions.
Funding AgencyGrant Number
NSF Graduate Research FellowshipUNSPECIFIED
Air Force Office of Scientific Research (AFOSR)FA9550-14-1-0060
Record Number:CaltechAUTHORS:20200205-074759124
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:101128
Deposited By: Tony Diaz
Deposited On:05 Feb 2020 15:53
Last Modified:05 Feb 2020 15:53

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