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Design and application of stationary phase combinatorial promoters

Hsiao, Victoria and Cheng, Aileen and Murray, Richard M. (2016) Design and application of stationary phase combinatorial promoters. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20200205-075449007

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Abstract

Current bacterial synthetic circuits rely on the fast dilution and high protein expression that occurs during exponential phase. However, constant exponential phase is both difficult to ensure in a lab environment and almost certainly impractical in any natural setting. Here, we characterize the performance of 13 E. coli native σ38 promoters, as well as a previously identified σ38 consensus promoter. We then make tetO combinatorial versions of the three strongest promoters to allow for inducible delayed expression. The design of these combinatorial promoters allows for design of circuits with inducible stationary phase activity that can be used for phase-dependent delays in dynamic circuits or spatial partitioning of biofilms.


Item Type:Report or Paper (Discussion Paper)
ORCID:
AuthorORCID
Hsiao, Victoria0000-0001-9297-1522
Murray, Richard M.0000-0002-5785-7481
Additional Information:VH is supported by the Department of Defense (DoD) through the National Defense Science & Engineering Graduate Fellowship (NDSEG) Program. Research supported in part by the Institute for Collaborative Biotechnologies through grant W911NF-09-0001 from the U.S. Army Research Office. The content of the information does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred.
Funders:
Funding AgencyGrant Number
Department of DefenseUNSPECIFIED
National Defense Science and Engineering Graduate (NDSEG) FellowshipUNSPECIFIED
Army Research Office (ARO)W911NF-09-0001
Subject Keywords:Stationary phase, combinatorial promoters, biofilms
Record Number:CaltechAUTHORS:20200205-075449007
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200205-075449007
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:101129
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:06 Feb 2020 18:03
Last Modified:06 Feb 2020 18:03

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