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Lateral Gene Transfer Drives Metabolic Flexibility in the Anaerobic Methane-Oxidizing Archaeal Family Methanoperedenaceae

Leu, Andy O. and McIlroy, Simon J. and Ye, Jun and Parks, Donovan H. and Orphan, Victoria J. and Tyson, Gene W. (2020) Lateral Gene Transfer Drives Metabolic Flexibility in the Anaerobic Methane-Oxidizing Archaeal Family Methanoperedenaceae. mBio, 11 (3). Art. No. e01325-20. ISSN 2150-7511. PMCID PMC7327174. https://resolver.caltech.edu/CaltechAUTHORS:20200207-102104518

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Abstract

Anaerobic oxidation of methane (AOM) is an important biological process responsible for controlling the flux of methane into the atmosphere. Members of the archaeal family Methanoperedenaceae (formerly ANME-2d) have been demonstrated to couple AOM to the reduction of nitrate, iron, and manganese. Here, comparative genomic analysis of 16 Methanoperedenaceace metagenome-assembled genomes (MAGs), recovered from diverse environments, revealed novel respiratory strategies acquired through lateral gene transfer (LGT) events from diverse archaea and bacteria. Comprehensive phylogenetic analyses suggests that LGT has allowed members of the Methanoperedenaceae to acquire genes for the oxidation of hydrogen and formate, and the reduction of arsenate, selenate and elemental sulfur. Numerous membrane-bound multi-heme c type cytochrome complexes also appear to have been laterally acquired, which may be involved in the direct transfer of electrons to metal oxides, humics and syntrophic partners.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1128/mBio.01325-20DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc7327174/PubMed CentralArticle
https://doi.org/10.1101/2020.02.06.936641DOIDiscussion Paper
ORCID:
AuthorORCID
Leu, Andy O.0000-0002-9882-9364
McIlroy, Simon J.0000-0003-3749-8730
Parks, Donovan H.0000-0001-6662-9010
Orphan, Victoria J.0000-0002-5374-6178
Tyson, Gene W.0000-0001-8559-9427
Alternate Title:Lateral gene transfer drives metabolic flexibility in the anaerobic methane oxidising archaeal family Methanoperedenaceae
Additional Information:© 2020 Leu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Received 18 May 2020; Accepted 27 May 2020; Published 30 June 2020. This work was supported by the Australian Research Council (ARC) (grant FT170100070) and the U.S. Department of Energy’s Office of Biological Environmental Research (grant DE-SC0016469). A.O.L. was supported by an ARC Australian Postgraduate Award, and S.J.M. was partly supported by an ARC Future Fellowship (FT190100211). We thank the AWMC team, particularly Shihu Hu and Zhiguo Yuan, for their ongoing collaboration working on various “Ca. Methanoperedens” enrichments. We have nothing to disclose. Data availability.The genomes assembled in this study have been deposited in the NCBI database under the accession numbers SAMN10961276 to SAMN10961283.
Funders:
Funding AgencyGrant Number
Australian Research CouncilFT170100070
Department of Energy (DOE)DE-SC0016469
Australian Research CouncilFT190100211
Subject Keywords:ANME, AOM, comparative genomics, methane, Methanoperedenaceae
Issue or Number:3
PubMed Central ID:PMC7327174
Record Number:CaltechAUTHORS:20200207-102104518
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200207-102104518
Official Citation:Lateral Gene Transfer Drives Metabolic Flexibility in the Anaerobic Methane-Oxidizing Archaeal Family Methanoperedenaceae. Andy O. Leu, Simon J. McIlroy, Jun Ye, Donovan H. Parks, Victoria J. Orphan, Gene W. Tyson. mBio Jun 2020, 11 (3) e01325-20; DOI: 10.1128/mBio.01325-20
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:101179
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:10 Feb 2020 15:26
Last Modified:23 Dec 2020 23:39

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