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A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms and flies

Borden, Philip M. and Shivange, Amol V. and Lester, Henry A. and Fan, Chengcheng and Rees, Douglas C. (2020) A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms and flies. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20200210-132609996

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Abstract

Here we design and optimize a genetically encoded fluorescent indicator, iAChSnFR, for the ubiquitous neurotransmitter acetylcholine, based on a bacterial periplasmic binding protein. iAChSnFR shows large fluorescence changes, rapid rise and decay kinetics, and insensitivity to most cholinergic drugs. iAChSnFR revealed large transients in a variety of slice and in vivo preparations in mouse, fish, fly and worm. iAChSnFR will be useful for the study of acetylcholine in all organisms.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/2020.02.07.939504DOIDiscussion Paper
ORCID:
AuthorORCID
Lester, Henry A.0000-0002-5470-5255
Fan, Chengcheng0000-0003-4213-5758
Rees, Douglas C.0000-0003-4073-1185
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. bioRxiv preprint first posted online Feb. 8, 2020. We would like to thank Deepika Walpita (Janelia) for rat neuronal culture, and Kim Ritola (Janelia) for making AAV virus. We thank Jens T. Kaiser at the Caltech Molecular Observatory and the beamline staff at SSRL 12-2 for their support during data collection. DD and HK thank Dr. Martin Fuhrmann (DZNE, Bonn, Germany) for help with virus injections. This work was supported by the Howard Hughes Medical Institute. JJZ is supported by NIH NS104670, PZ by an Alzheimer’s Association research fellowship, CPF by NIH NS95809, PKZ by a 2019 International Science and Engineering Fair award, WSZ by a 2019 Harrison undergraduate research award, and GW by Epilepsy Foundation postdoctoral fellowship No. 310443. JJZ is the Radboud Professor and Sir Yue-Kong Pao Chair Professor. HAL and AVS are supported by NIH DA037161, NIH DA043829, California Tobacco-Related Disease Research Program 23XT-0007 and 27IP-0057, and NARSAD. JSD is supported by R01-GM095674. JFC is supported by NIH DA022340, and AF by a STAR-PREP fellowship. LL is supported by a National Natural Science Foundation of China grant NSFC81771284. We gratefully acknowledge the Gordon and Betty Moore Foundation and the Beckman Institute for their generous support of the Molecular Observatory at Caltech. Use of the Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, is supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences under Contract No. DE-AC02-76SF00515. The SSRL Structural Molecular Biology Program is supported by the DOE Office of Biological and Environmental Research, and by the NIH, NIGMS (P41GM103393). BSK is supported by NIH (NS111583, DA047444, MH104069). DD and HK are supported by DFG (SPP1757, SFB1089, DI853/3-5) and BONFOR to DD. WBG is supported by NIH R01 NS047325. ES is supported by JSPS KAKENHI #15KK0340. Author contributions: P.M.B., P.Z., H.A.L., J.J.Z., and L.L.L. led the project. P.M.B., A.V.S., J.S.M., H.A.L., and L.L.L. performed protein engineering. P.M.B., A.V.S., and J.S.M. performed biochemical characterization. P.M.B., J.S.M., C.F., D.C.R. acquired and refined crystal structures. P.M.B., A.A., and R.P. performed spectroscopy. P.M.B. and J.S.M. performed HEK293 and cultured neuron experiments. P.Z. and Y.Z. screened sensor variants in brain slice. P.Z., P.K.Z., Y.Z., W.S.Z., G.W., and B.X. acquired and analyzed nanoscopic imaging data. P.Z., Y.Z., L.G., G.-X.Z., K.G., and L.L. made Sindbis and lentivirus constructs and live virus. P.Z. and Y.Z. performed other slice characterization. H.K. and D.D. performed hippocampal stratum radiatum slice characterization. E.S. and B.S.K. performed hippocampal astrocyte slice characterization. Y.C., A.G.Y., and C.P.F. performed striatal slice characterization. J.S.D. acquired and analyzed the C. elegans data. C.D. and V.J. acquired and analyzed the Drosophila data. M.T. and M.K. acquired and analyzed the zebrafish data. O.N. and K.P. did SLAP imaging and experiments on the effects of anesthesia. J.C. and W.-B.G. did motor cortex experiments. A.F. and J.F.C. did hippocampal reward experiments. P.M.B., H.A.L., J.J.Z., and L.L.L. wrote the paper, with help from all authors. Code availability: All analysis code used in this study is available upon request. Data availability: All data from this study is available upon request. Accession codes: All constructs have been deposited at Addgene (#137950-137959). Sequences have been deposited in Genbank. PDB accession code for iAChSnFR0.4 is 6URU. PDB accession code for the binding protein-only component of iAChSnFR is 6V1R. AAV virus is available from Addgene. The authors declare no competing financial interests.
Funders:
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
NIHNS104670
Alzheimer's AssociationUNSPECIFIED
NIHNS95809
Harvard UniversityUNSPECIFIED
University of VirginiaUNSPECIFIED
Epilepsy Foundation of America310443
NIHDA037161
NIHDA043829
California Tobacco-Related Disease Research Program23XT-0007
California Tobacco-Related Disease Research Program27IP-0057
Brain and Behavior Research FoundationUNSPECIFIED
NIHR01-GM095674
NIHDA022340
University of MarylandUNSPECIFIED
National Natural Science Foundation of China81771284
Gordon and Betty Moore FoundationUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
Department of Energy (DOE)DE-AC02-76SF00515
NIHP41GM103393
NIHNS111583
NIHDA047444
NIHMH104069
Deutsche Forschungsgemeinschaft (DFG)SPP1757
Deutsche Forschungsgemeinschaft (DFG)SFB1089
Deutsche Forschungsgemeinschaft (DFG)DI853/3-5
BONFOR-ForschungsförderprogrammUNSPECIFIED
NIHR01 NS047325
Japan Society for the Promotion of Science (JSPS)15KK0340
Record Number:CaltechAUTHORS:20200210-132609996
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200210-132609996
Official Citation:A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms and flies. Phil M Borden, Peng Zhang, Amol V Shivange, Jonathan S Marvin, Joseph Cichon, Chuntao Dan, Kaspar Podgorski, Antonio Figueiredo, Ondrej Novak, Masashi Tanimoto, Eiji Shigetomi, Mark A Lobas, Hyuntae Kim, Paula K Zhu, Yajun Zhang, W. Sharon Zheng, Chengcheng Fan, Guangfu Wang, Bowen Xiang, Li Gan, Guang-Xian Zhang, Kaiming Guo, Li Lin, Yuan Cai, Andrew Yee, Abhi Aggarwal, Christopher P Ford, Douglas C Rees, Dirk Dietrich, BALJIT S KHAKH, Jeremy S Dittman, Wen-Biao Gan, Minoru S Koyama, Vivek Jayaraman, Joseph F Cheer, Henry A Lester, J. Julius Zhu, Loren L Looger. bioRxiv 2020.02.07.939504; doi: https://doi.org/10.1101/2020.02.07.939504
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:101206
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:10 Feb 2020 21:40
Last Modified:10 Feb 2020 21:40

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