Zhu, Meng and Wang, Peizhe and Handford, Charlotte and Na, Jie and Zernicka-Goetz, Magdalena (2020) Transcriptional control of apical protein clustering drives de novo cell polarity establishment in the early mouse embryo. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20200212-084819179
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Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20200212-084819179
Abstract
The establishment of cell polarity de novo in the early mammalian embryo triggers the transition from totipotency to differentiation to generate embryonic and extra-embryonic lineages. However, the molecular mechanisms governing the timing of cell polarity establishment remain unknown. Here, we identify stage-dependent transcription of Tfap2c and Tead4 as well as Rho GTPase signaling as key for the onset of cell polarization. Importantly, advancing their activity can induce precocious cell polarization and ectopic lineage differentiation in a cell-autonomous manner. Moreover, we show that the asymmetric clustering of apical proteins, regulated by Tfap2c-Tead4, and not actomyosin flow, mediates apical protein polarization. These findings identify the long-sought mechanism for the onset of polarization and the first lineage segregation in the mouse embryo.
Item Type: | Report or Paper (Discussion Paper) | ||||||||
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Additional Information: | The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. We are grateful to David Glover and Marta Shahbazi for valuable comments on the manuscript; Ed Munro for helpful discussion of the project; Stavros Malas for providing the Gata3-GFP transgenic line. This work was supported by Wellcome Trust (098287/Z/12/Z), ERC (669198) and Leverhulme Trust (RPG-2018-085) grants to M.Z.G. Author contributions: Conceptualization: M.Z and M.Z.G.; Investigation: M.Z., P.W., C.H; Writing: M.Z and M.Z.G.; Supervision: M.Z.G., N.J. The authors declare no competing interests. | ||||||||
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Record Number: | CaltechAUTHORS:20200212-084819179 | ||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20200212-084819179 | ||||||||
Official Citation: | Transcriptional control of apical protein clustering drives de novo cell polarity establishment in the early mouse embryo. Meng Zhu, Peizhe Wang, Charlotte E. Handford, Jie Na, Magdalena Zernicka-Goetz. bioRxiv 2020.02.10.942201; doi: https://doi.org/10.1101/2020.02.10.942201 | ||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||
ID Code: | 101233 | ||||||||
Collection: | CaltechAUTHORS | ||||||||
Deposited By: | Tony Diaz | ||||||||
Deposited On: | 12 Feb 2020 17:35 | ||||||||
Last Modified: | 12 Feb 2020 17:35 |
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