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Systematic Design and Implementation of a Novel Synthetic Fold-Change Detector Biocircuit In Vivo

Guo, Shaobin and Hori, Yutaka and Murray, Richard (2015) Systematic Design and Implementation of a Novel Synthetic Fold-Change Detector Biocircuit In Vivo. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20200214-103858802

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Abstract

Biological signaling systems not only detect the absolute levels of the signals, but are also able to sense the fold-changes of the signals. The ability to detect fold-changes provides a powerful tool for biological organisms to adapt to the changes in environment. Here we present the first novel synthetic fold-change detector (FCD) circuit built from ground up in vivo. We systematically designed the FCD circuit in silico, prototyped it in cell-free transcription-translation platform (TX-TL), and eventually implemented it in E. coli cells. We were able to show that the FCD circuit can not only generate pulse-like behavior in response to input, but also produce the same pulse response with inputs of the same fold-change, despite of different absolute signal levels.


Item Type:Report or Paper (Discussion Paper)
ORCID:
AuthorORCID
Guo, Shaobin0000-0001-9736-4078
Hori, Yutaka0000-0002-3253-4985
Murray, Richard0000-0002-5785-7481
Record Number:CaltechAUTHORS:20200214-103858802
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200214-103858802
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:101294
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 Feb 2020 18:52
Last Modified:14 Feb 2020 18:52

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