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ESEEM studies of succinate:ubiquinone reductase from Paracoccus denitrificans

Hung, Shao-Ching and Grant, Christopher V. and Peloquin, Jeffrey M. and Waldeck, A. Reginald and Britt, R. David and Chan, Sunney I. (2000) ESEEM studies of succinate:ubiquinone reductase from Paracoccus denitrificans. Journal of Biological Inorganic Chemistry, 5 (5). pp. 593-602. ISSN 0949-8257. https://resolver.caltech.edu/CaltechAUTHORS:20200219-114918387

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Abstract

Electron spin-echo envelope modulation (ESEEM) spectroscopy has been performed in order to obtain structural information about the environment of the reduced [2Fe-2S] cluster (S-1 center), the oxidized [3Fe-4S] cluster (S-3 center), and the flavin semiquinone radical in purified succinate:ubiquinone reductase from Paracoccus denitrificans. Spectral simulations of the ESEEM data from the reduced [2Fe-2S] yielded nuclear quadrupole interaction parameters that are indicative of peptide nitrogens. We also observed a weak interaction between the oxidized [3Fe-4S] cluster and a peptide ¹⁴N. There was no evidence for coordination of any of the Fe atoms to ¹⁴N atoms of imidazole rings. The ESEEM data from the flavin semiquinone radical were more complicated. Here, evidence was obtained for interactions between the unpaired electron and only the two nitrogen atoms in the flavin ring.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1007/s007750000142DOIArticle
https://rdcu.be/b1Vw2PublisherFree ReadCube access
ORCID:
AuthorORCID
Grant, Christopher V.0000-0003-1692-5414
Britt, R. David0000-0003-0889-8436
Chan, Sunney I.0000-0002-5348-2723
Additional Information:© 2000 SBIC. Received 29 July 1999; Accepted 16 May 2000; Published online: 27 July 2000. We would like to thank Dr. Chrisopher J. Bender and Biotechnology Resource in Pulsed EPR Spectroscopy at Albert Einstein College of Medicine for providing us with a copy of their spectral simulation program. This work was supported by the NIH grants GM22432 to (S.I.C.) and GM48242 (to R.D.B.) from the U.S. Public Health Service.
Funders:
Funding AgencyGrant Number
NIHGM22432
NIHGM48242
Subject Keywords:2Fe-2S cluster; 4Fe-4S cluster; 3Fe-4S cluster; Flavin semiquinone radical; Electron spin-echo envelope modulation spectroscopy
Issue or Number:5
Record Number:CaltechAUTHORS:20200219-114918387
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200219-114918387
Official Citation:Hung, S., Grant, C., Peloquin, J. et al. ESEEM studies of succinate:ubiquinone reductase from Paracoccus denitrificans . J. Biol. Inorg. Chem. 5, 593–602 (2000). https://doi.org/10.1007/s007750000142
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:101384
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:19 Feb 2020 20:56
Last Modified:19 Feb 2020 20:56

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