Yokobayashi, Yohei and Arnold, Frances H. (2005) A Dual Selection Module for Directed Evolution of Genetic Circuits. Natural Computing, 4 (3). pp. 245-254. ISSN 1567-7818. doi:10.1007/s11047-004-7442-x. https://resolver.caltech.edu/CaltechAUTHORS:20200221-125714643
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20200221-125714643
Abstract
We have developed a genetic selection module which enables us to select for positive and negative expression of a gene by controlling the expression of two antibiotic resistance genes. This module can be used to tune the performance of genetic circuits that yield simple ON/OFF gene expression outputs depending on the environment (input). We have demonstrated the utility of the module by selecting a genetic inverter from a 200 fold excess of nonfunctional inverters in two rounds.
Item Type: | Article | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Related URLs: |
| |||||||||
ORCID: |
| |||||||||
Additional Information: | © 2005 Springer. We thank Tim Palzkill for providing pTP154 from which bli gene was engineered and the National Science Foundation (Award # 0130613) for financial support. | |||||||||
Funders: |
| |||||||||
Subject Keywords: | cellular computation, directed evolution, genetic circuits, selection, synthetic biology, synthetic gene circuits | |||||||||
Issue or Number: | 3 | |||||||||
DOI: | 10.1007/s11047-004-7442-x | |||||||||
Record Number: | CaltechAUTHORS:20200221-125714643 | |||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20200221-125714643 | |||||||||
Official Citation: | Yokobayashi, Y., Arnold, F.H. A Dual Selection Module for Directed Evolution of Genetic Circuits. Nat Comput 4, 245–254 (2005). https://doi.org/10.1007/s11047-004-7442-x | |||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
ID Code: | 101461 | |||||||||
Collection: | CaltechAUTHORS | |||||||||
Deposited By: | Tony Diaz | |||||||||
Deposited On: | 21 Feb 2020 21:27 | |||||||||
Last Modified: | 16 Nov 2021 18:02 |
Repository Staff Only: item control page