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A Dual Selection Module for Directed Evolution of Genetic Circuits

Yokobayashi, Yohei and Arnold, Frances H. (2005) A Dual Selection Module for Directed Evolution of Genetic Circuits. Natural Computing, 4 (3). pp. 245-254. ISSN 1567-7818. doi:10.1007/s11047-004-7442-x. https://resolver.caltech.edu/CaltechAUTHORS:20200221-125714643

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Abstract

We have developed a genetic selection module which enables us to select for positive and negative expression of a gene by controlling the expression of two antibiotic resistance genes. This module can be used to tune the performance of genetic circuits that yield simple ON/OFF gene expression outputs depending on the environment (input). We have demonstrated the utility of the module by selecting a genetic inverter from a 200 fold excess of nonfunctional inverters in two rounds.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1007/s11047-004-7442-xDOIArticle
https://rdcu.be/b11C6PublisherFree ReadCube access
ORCID:
AuthorORCID
Yokobayashi, Yohei0000-0002-2417-1934
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2005 Springer. We thank Tim Palzkill for providing pTP154 from which bli gene was engineered and the National Science Foundation (Award # 0130613) for financial support.
Funders:
Funding AgencyGrant Number
NSFCCF-0130613
Subject Keywords:cellular computation, directed evolution, genetic circuits, selection, synthetic biology, synthetic gene circuits
Issue or Number:3
DOI:10.1007/s11047-004-7442-x
Record Number:CaltechAUTHORS:20200221-125714643
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200221-125714643
Official Citation:Yokobayashi, Y., Arnold, F.H. A Dual Selection Module for Directed Evolution of Genetic Circuits. Nat Comput 4, 245–254 (2005). https://doi.org/10.1007/s11047-004-7442-x
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:101461
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:21 Feb 2020 21:27
Last Modified:16 Nov 2021 18:02

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