Yokobayashi, Yohei and Arnold, Frances H. (2005) A Dual Selection Module for Directed Evolution of Genetic Circuits. Natural Computing, 4 (3). pp. 245-254. ISSN 1567-7818. doi:10.1007/s11047-004-7442-x. https://resolver.caltech.edu/CaltechAUTHORS:20200221-125714643
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Abstract
We have developed a genetic selection module which enables us to select for positive and negative expression of a gene by controlling the expression of two antibiotic resistance genes. This module can be used to tune the performance of genetic circuits that yield simple ON/OFF gene expression outputs depending on the environment (input). We have demonstrated the utility of the module by selecting a genetic inverter from a 200 fold excess of nonfunctional inverters in two rounds.
| Item Type: | Article | |||||||||
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| Additional Information: | © 2005 Springer. We thank Tim Palzkill for providing pTP154 from which bli gene was engineered and the National Science Foundation (Award # 0130613) for financial support. | |||||||||
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| Subject Keywords: | cellular computation, directed evolution, genetic circuits, selection, synthetic biology, synthetic gene circuits | |||||||||
| Issue or Number: | 3 | |||||||||
| DOI: | 10.1007/s11047-004-7442-x | |||||||||
| Record Number: | CaltechAUTHORS:20200221-125714643 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20200221-125714643 | |||||||||
| Official Citation: | Yokobayashi, Y., Arnold, F.H. A Dual Selection Module for Directed Evolution of Genetic Circuits. Nat Comput 4, 245–254 (2005). https://doi.org/10.1007/s11047-004-7442-x | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 101461 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Tony Diaz | |||||||||
| Deposited On: | 21 Feb 2020 21:27 | |||||||||
| Last Modified: | 16 Nov 2021 18:02 |
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