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Enzyme Stabilization by Directed Evolution

Gershenson, Anne and Arnold, Frances H. (2000) Enzyme Stabilization by Directed Evolution. In: Genetic Engineering: Principles and Methods. Genetic Engineering. No.22. Springer , Boston, MA, pp. 55-76. ISBN 978-1-4613-6884-7.

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Engineered enzymes are widely used in industrial processes, where they provide distinct advantages over the naturally-occurring ones (1, 2). Engineering a useful enzyme is particularly difficult when the target functions—for example, the environment in which it must work or the substrates it must accept--differ significantly from the natural ones. ‘Rational ‘design approaches depend on detailed knowledge of enzyme structure and function. Unfortunately, the three-dimensional conformations of most enzymes are unknown, and, even when a structure is available, the relationship of structure to function is often not apparent. Further complicating the design problem is the fact that the effects of mutations can be propagated over long distances, such that mutations far from the active site can affect catalytic activity (3–5) and even single mutations can generate large structural changes (6, 7).

Item Type:Book Section
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Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2000 Springer Science+Business Media New York. The authors wish to thank Luis Garcia for assistance in characterizing esterase mutants and the Caltech MURF program for his support. The authors also thank Dr. Kentaro Miyazaki and Dr. Oliver May for access to their results prior to publication. Support from ThermoGen and the Army Research Office is gratefully acknowledged.
Funding AgencyGrant Number
Army Research Office (ARO)UNSPECIFIED
Caltech Minority Undergraduate Research Fellowship (MURF)UNSPECIFIED
Subject Keywords:Direct Evolution; Enzyme Stabilization; Random Mutagenesis; Mutant Library; Positive Mutant
Series Name:Genetic Engineering
Issue or Number:22
Record Number:CaltechAUTHORS:20200221-130840993
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:101463
Deposited By: Tony Diaz
Deposited On:21 Feb 2020 21:28
Last Modified:16 Nov 2021 18:02

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