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High-contrast, fast chemical imaging by coherent Raman scattering using a self-synchronized two-colour fibre laser

Kong, Cihang and Pilger, Christian and Hachmeister, Henning and Wei, Xiaoming and Cheung, Tom H. and Lai, Cora S. W. and Lee, Nikki P. and Tsia, Kevin K. and Wong, Kenneth K. Y. and Huser, Thomas (2020) High-contrast, fast chemical imaging by coherent Raman scattering using a self-synchronized two-colour fibre laser. Light: Science & Applications, 9 . Art. No. 25. ISSN 2047-7538. PMCID PMC7039946. https://resolver.caltech.edu/CaltechAUTHORS:20200224-152010523

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Abstract

Coherent Raman scattering (CRS) microscopy is widely recognized as a powerful tool for tackling biomedical problems based on its chemically specific label-free contrast, high spatial and spectral resolution, and high sensitivity. However, the clinical translation of CRS imaging technologies has long been hindered by traditional solid-state lasers with environmentally sensitive operations and large footprints. Ultrafast fibre lasers can potentially overcome these shortcomings but have not yet been fully exploited for CRS imaging, as previous implementations have suffered from high intensity noise, a narrow tuning range and low power, resulting in low image qualities and slow imaging speeds. Here, we present a novel high-power self-synchronized two-colour pulsed fibre laser that achieves excellent performance in terms of intensity stability (improved by 50 dB), timing jitter (24.3 fs), average power fluctuation (<0.5%), modulation depth (>20 dB) and pulse width variation (<1.8%) over an extended wavenumber range (2700–3550 cm⁻¹). The versatility of the laser source enables, for the first time, high-contrast, fast CRS imaging without complicated noise reduction via balanced detection schemes. These capabilities are demonstrated in this work by imaging a wide range of species such as living human cells and mouse arterial tissues and performing multimodal nonlinear imaging of mouse tail, kidney and brain tissue sections by utilizing second-harmonic generation and two-photon excited fluorescence, which provides multiple optical contrast mechanisms simultaneously and maximizes the gathered information content for biological visualization and medical diagnosis. This work also establishes a general scenario for remodelling existing lasers into synchronized two-colour lasers and thus promotes a wider popularization and application of CRS imaging technologies.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1038/s41377-020-0259-2DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039946PubMed CentralArticle
ORCID:
AuthorORCID
Kong, Cihang0000-0002-8551-6959
Wei, Xiaoming0000-0001-6136-5335
Tsia, Kevin K.0000-0002-6394-9657
Wong, Kenneth K. Y.0000-0003-3668-3539
Huser, Thomas0000-0003-2348-7416
Additional Information:© 2020 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Received 14 May 2019; Revised 23 January 2020; Accepted 09 February 2020; Published 24 February 2020. The authors thank Dr. Gerd Wiebusch for help with selecting and purchasing the optical components as well as his helpful comments and advice during the instrument development process; Prof. Barbara P. Chan for discussing and analysing the imaging results of the tissue samples; and Prof. Walter Pfeiffer for commenting on the manuscript. This work was supported by the Germany/Hong Kong Joint Research Scheme sponsored by the Research Grants Council of Hong Kong and the Germany Academic Exchange Service of Germany (G-HKU708/14, DAAD-57138104), the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie Grant Agreement No. 766181, project “DeLIVER”, the Research Grants Council of the Hong Kong Special Administrative Region, China (HKU 17205215, CityU T42-103/16-N, E-HKU701/17, and HKU C7047-16G), and the National Natural Science Foundation of China (N_HKU712/16); Innovation and Technology Fund (GHP/050/14GD). We acknowledge support for the Article Processing Charge by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University. Author Contributions: X.W., K.K.Y.W. and T.H. conceived the idea. X.W. and C.K. designed the two-colour pulsed fibre laser system. C.K., C.P. and H.H. built and characterized the laser system. C.K., C.P. and H.H. built the laser scanning microscope and performed the imaging experiments. C.P. and H.H. analyzed the imaging data. T.H.C., C.S.W.L. and N.P.L. prepared the biological samples. K.K.T., K.K.Y.W. and T.H. obtained funding for this research. All authors wrote and commented on the manuscript. The authors declare no competing interests.
Funders:
Funding AgencyGrant Number
Research Grants Council of Hong KongG-HKU708/14
Deutscher Akademischer Austauschdienst (DAAD)DAAD-57138104
Marie Curie Fellowship766181
Research Grants Council of the Hong Kong Special Administrative RegionHKU 17205215
Research Grants Council of the Hong Kong Special Administrative RegionCityU T42-103/16-N
Research Grants Council of the Hong Kong Special Administrative RegionE-HKU701/17
Research Grants Council of the Hong Kong Special Administrative RegionHKU C7047-16G
National Natural Science Foundation of ChinaN_HKU712/16
National Natural Science Foundation of ChinaGHP/050/14GD
Deutsche Forschungsgemeinschaft (DFG)UNSPECIFIED
Bielefeld UniversityUNSPECIFIED
PubMed Central ID:PMC7039946
Record Number:CaltechAUTHORS:20200224-152010523
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200224-152010523
Official Citation:Kong, C., Pilger, C., Hachmeister, H. et al. High-contrast, fast chemical imaging by coherent Raman scattering using a self-synchronized two-colour fibre laser. Light Sci Appl 9, 25 (2020). https://doi.org/10.1038/s41377-020-0259-2
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:101508
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:24 Feb 2020 23:30
Last Modified:06 Mar 2020 15:53

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