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Fighting against fast speckle decorrelation for light focusing inside live tissue by photon frequency shifting

Yang, Jiamiao and Li, Lei and Li, Jingwei and Cheng, Zhongtao and Liu, Yan and Wang, Lihong V. (2020) Fighting against fast speckle decorrelation for light focusing inside live tissue by photon frequency shifting. ACS Photonics, 7 (3). pp. 837-844. ISSN 2330-4022. PMCID PMC8188831. doi:10.1021/acsphotonics.0c00027.

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[img] PDF (Mathematical description of light focusing inside tissue by binary-phase DOPC. S2: Enhancing the quality of the light focusing by fast DOPC. S3: Mathematical description of light focusing inside tissue by PFM-DOPC. S4: Simulation parameters of the...) - Supplemental Material
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Light focusing inside live tissue by digital optical phase conjugation (DOPC) has drawn increasing interest due to its potential biomedical applications in optogenetics, microsurgery, phototherapy, and deep-tissue imaging. However, fast physiological motions in a live animal, including blood flow and respiratory motions, produce undesired photon perturbation and thus inevitably deteriorate the performance of light focusing. Here, we develop a photon-frequency-shifting DOPC method to fight against fast physiological motions by switching the states of a guide star at a distinctive frequency. Therefore, the photons tagged by the guide star are well detected at the specific frequency, separating them from the photons perturbed by fast motions. Light focusing was demonstrated in both phantoms in vitro and mice in vivo with substantially improved focusing contrast. This work puts a new perspective on light focusing inside live tissue and promises wide biomedical applications.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Yang, Jiamiao0000-0003-0006-8411
Li, Lei0000-0001-6164-2646
Li, Jingwei0000-0003-4927-1066
Cheng, Zhongtao0000-0002-9862-2873
Liu, Yan0000-0002-5837-4908
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2020 American Chemical Society. Received: January 7, 2020; Published: February 26, 2020. This work was financially supported by the National Institutes of Health (NIH), Grants CA186567 (NIH Director’s Transformative Research Award), NS090579, and NS099717. The authors declare the following competing financial interest(s): L. V. Wang has a financial interest in Microphotoacoustics, Inc., CalPACT, LLC, and Union Photoacoustic Technologies, Ltd., which, however, did not support this work. The other authors declare no competing financial interests.
Funding AgencyGrant Number
Subject Keywords:Wavefront shaping; Digital optical phase conjugation; Dynamic scattering medium; Guide star; Spatial light modulator; Lock-in detection; Angular-spectrum model
Issue or Number:3
PubMed Central ID:PMC8188831
Record Number:CaltechAUTHORS:20200226-132116150
Persistent URL:
Official Citation:Fighting against Fast Speckle Decorrelation for Light Focusing inside Live Tissue by Photon Frequency Shifting. Jiamiao Yang, Lei Li, Jingwei Li, Zhongtao Cheng, Yan Liu, and Lihong V. Wang. ACS Photonics 2020 7 (3), 837-844; DOI: 10.1021/acsphotonics.0c00027
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:101576
Deposited By: Tony Diaz
Deposited On:26 Feb 2020 21:36
Last Modified:11 Jun 2021 17:04

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