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Pectin homogalacturonan nanofilament expansion drives morphogenesis in plant epidermal cells

Haas, Kalina T. and Wightman, Raymond and Meyerowitz, Elliot M. and Peaucelle, Alexis (2020) Pectin homogalacturonan nanofilament expansion drives morphogenesis in plant epidermal cells. Science, 367 (6481). pp. 1003-1007. ISSN 0036-8075. doi:10.1126/science.aaz5103. https://resolver.caltech.edu/CaltechAUTHORS:20200227-140409173

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Abstract

The process by which plant cells expand and gain shape has presented a challenge for researchers. Current models propose that these processes are driven by turgor pressure acting on the cell wall. Using nanoimaging, we show that the cell wall contains pectin nanofilaments that possess an intrinsic expansion capacity. Additionally, we use growth models containing such structures to show that a complex plant cell shape can derive from chemically induced local and polarized expansion of the pectin nanofilaments without turgor-driven growth. Thus, the plant cell wall, outside of the cell itself, is an active participant in shaping plant cells. Extracellular matrix function may similarly guide cell shape in other kingdoms, including Animalia.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1126/science.aaz5103DOIArticle
https://science.sciencemag.org/content/367/6481/1003/suppl/DC1PublisherSupplementary Materials
ORCID:
AuthorORCID
Wightman, Raymond0000-0003-1295-4875
Meyerowitz, Elliot M.0000-0003-4798-5153
Peaucelle, Alexis0000-0002-8582-6500
Additional Information:© 2020 American Association for the Advancement of Science. This is an article distributed under the terms of the Science Journals Default License. Received 15 September 2019; accepted 24 January 20. The dSTORM was performed at the Medical Research Council Laboratory of Molecular Biology, Cambridge, and we thank N. Barry and J. Howe for their support. We thank A. Gurzadyan for the critical evaluation of our FEM model and H. Höfte for help with the fundraising and discussion of the results. Funding: A.P. has received support from the French National Research Agency (ANR) GoodVibration ANR-17-CE13-0007 and the European Union in the framework of the Marie-Curie FP7 COFUND People Program, through the award of an AgreenSkills+ fellowship (under grant agreement 201310). The Institut Jean-Pierre Bourgin (IJPB) benefits from the support of Saclay Plant Sciences-SPS (ANR-17-EUR-0007). The Microscopy Facility at the Sainsbury Laboratory is supported by the Gatsby Charitable Foundation, and work at the Sainsbury Laboratory was supported by the Gatsby Charitable Foundation (through fellowship GAT3395/DAA). The Meyerowitz Laboratory has support from the Howard Hughes Medical Institute. This work has benefited from the support of IJPB's Plant Observatory technological platforms. Author contributions: K.T.H.: software and visualization; K.T.H. and A.P.: investigation, methodology, and formal analysis; R.W. carried out the cryo-SEM; A.P., E.M.M., K.T.H., and R.W.: writing, original draft, and writing, review and editing; A.P. with the help of other authors: conceptualization. Competing interests: E.M.M. is a member of the Management Board of the Sainsbury Laboratory at Cambridge University. The authors declare no other competing interests. Data and materials availability: All data, codes, and materials used in this study are available. Please contact A.P. for the material request. All data are available in the manuscript, the supplementary materials, or at Mendeley (38). All codes used in this study are available on Zenodo (39–42).
Funders:
Funding AgencyGrant Number
Agence Nationale pour la Recherche (ANR)ANR-17-CE13-0007
Marie Curie Fellowship201310
Agence Nationale pour la Recherche (ANR)ANR-17-EUR-0007
Gatsby Charitable FoundationGAT3395/DAA
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:6481
DOI:10.1126/science.aaz5103
Record Number:CaltechAUTHORS:20200227-140409173
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200227-140409173
Official Citation:Pectin homogalacturonan nanofilament expansion drives morphogenesis in plant epidermal cells. BY KALINA T. HAAS, RAYMOND WIGHTMAN, ELLIOT M. MEYEROWITZ, ALEXIS PEAUCELLE. Science 28 Feb 2020: Vol. 367, Issue 6481, pp. 1003-1007 DOI: 10.1126/science.aaz5103
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:101625
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:27 Feb 2020 22:19
Last Modified:16 Nov 2021 18:04

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