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Changes in the physical state of DNA during the replication cycle

Rolfe, Ronald (1963) Changes in the physical state of DNA during the replication cycle. Proceedings of the National Academy of Sciences of the United States of America, 49 (3). pp. 386-392. ISSN 0027-8424.

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According to the original hypothesis of Watson and Crick (1) each strand of the DNA double helix serves as a template for the synthesis of a new complementary strand of opposite polarity. The observations of Meselson and Stahl (2) on the replication of DNA in E. coli, as well as the in vitro studies of Kornberg and co-workers (3), have been entirely consistent with this hypothesis. However, studies with highly purified preparations of DNA polymerase have served to underscore a problem which was raised by Watson and Crick in their original formulation (1). The strands of the DNA double helix are hydrogen bonded to each other and are not free to act as templates for replication. (2) DNA in its native double helical state is not an effective template for the synthesis of new DNA by highly purified preparations of polymerase (4,5).

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Additional Information:© 1963 by the National Academy of Sciences. Communicated by Renato Dulbecco, December 17, 1962. We wish to acknowledge the kind interest and helpful criticism of Professor Renato Dulbecco. P.C. Hanawalt and J.D. Smith provided helpful discussions. This investigation was supported in part by research grant No. E 5131 of U.S. Public Health Service and grant CVR-133 of the National Foundation.
Issue or Number:3
Record Number:CaltechAUTHORS:ROLpnas63
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10163
Deposited By: Tony Diaz
Deposited On:15 Apr 2008
Last Modified:03 Oct 2019 00:07

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