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Single-Molecule FRET of Intrinsically Disordered Proteins

Metskas, Lauren Ann and Rhoades, Elizabeth (2020) Single-Molecule FRET of Intrinsically Disordered Proteins. Annual Review of Physical Chemistry, 71 . pp. 391-414. ISSN 0066-426X.

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Intrinsically disordered proteins (IDPs) are now widely recognized as playing critical roles in a broad range of cellular functions as well as being implicated in diverse diseases. Their lack of stable secondary structure and tertiary interactions, coupled with their sensitivity to measurement conditions, stymies many traditional structural biology approaches. Single-molecule Förster resonance energy transfer (smFRET) is now widely used to characterize the physicochemical properties of these proteins in isolation and is being increasingly applied to more complex assemblies and experimental environments. This review provides an overview of confocal diffusion-based smFRET as an experimental tool, including descriptions of instrumentation, data analysis, and protein labeling. Recent papers are discussed that illustrate the unique capability of smFRET to provide insight into aggregation-prone IDPs, protein–protein interactions involving IDPs, and IDPs in complex experimental milieus.

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Metskas, Lauren Ann0000-0002-8073-6960
Additional Information:© 2020 Annual Reviews. Review in Advance first posted online on February 25, 2020. The authors are not aware of any affiliations, memberships, funding, or financial holdings that might be perceived as affecting the objectivity of this review.
Subject Keywords:single-molecule FRET, IDP, disordered protein, protein–protein interaction, aggregation, coupled binding and folding
Record Number:CaltechAUTHORS:20200303-133535471
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Official Citation:Single-Molecule FRET of Intrinsically Disordered Proteins Lauren Ann Metskas and Elizabeth Rhoades Annual Review of Physical Chemistry 2020 71:1, 391-414; doi: 10.1146/annurev-physchem-012420-104917
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:101683
Deposited By: Tony Diaz
Deposited On:04 Mar 2020 20:06
Last Modified:22 Apr 2020 17:20

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