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Inactivation of the srtA gene in Listeria monocytogenes inhibits anchoring of surface proteins and affects virulence

Bierne, Hélène and Mazmanian, Sarkis K. and Trost, Matthias and Pucciarelli, M. Graciela and Liu, Gwen and Dehoux, Pierre and Jänsch, Lothar and Garcia-del Portillo, Francisco and Schneewind, Olaf and Cossart, Pascale (2002) Inactivation of the srtA gene in Listeria monocytogenes inhibits anchoring of surface proteins and affects virulence. Molecular Microbiology, 43 (4). pp. 869-881. ISSN 0950-382X. doi:10.1046/j.1365-2958.2002.02798.x. https://resolver.caltech.edu/CaltechAUTHORS:20200309-161404837

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Abstract

During infection of their hosts, Gram‐positive bac‐teria express surface proteins that serve multiple biological functions. Surface proteins harbouring a C‐terminal sorting signal with an LPXTG motif are covalently linked to the cell wall peptidoglycan by a transamidase named sortase. Two genes encoding putative sortases, termed srtA and srtB, were identified in the genome of the intracellular pathogenic bacterium Listeria monocytogenes. Inactivation of srtA abolishes anchoring of the invasion protein InlA to the bacterial surface. It also prevents the proper sorting of several other peptidoglycan‐associated LPXTG proteins. Three were identified by a mass spectrometry approach. The ΔsrtA mutant strain is defective in entering epithelial cells, similar to a ΔinlA mutant. In contrast to a ΔinlA mutant, the ΔsrtA mutant is impaired for colonization of the liver and spleen after oral inoculation in mice. Thus, L. monocytogenes srtA is required for the cell wall anchoring of InlA and, presumably, for the anchoring of other LPXTG‐containing proteins that are involved in listerial infections.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1046/j.1365-2958.2002.02798.xDOIArticle
ORCID:
AuthorORCID
Mazmanian, Sarkis K.0000-0003-2713-1513
Additional Information:© 2002 Blackwell Science. Accepted 9 November, 2001. We thank P. Gounon for help with the immunogold labelling experiments, Veronique Villiers for help with plaque assays, Alain Zerdoun for technical assistance, and Didier Cabanes for helpful discussions and critical reading of this manuscript. Unpublished sequence data on B. anthracis were obtained from The Institute for Genomic Research website at http://www.tigr.org. S.K.M. is supported by the Predoctoral Training Program in Genetics (T32GM07104). Work in the laboratory of O.S. is supported by grant AI33987 from the National Institute of Allergy and Infectious Diseases, Infectious Disease Branch. Work in the laboratory of F.G.P is funded by the European Commission (contract QLG2-CT-1999-00932). Work in the laboratory of P.C. is supported by the Ministère de l’Education Nationale et de la Recherche Scientifique et Technique and the Pasteur Institute. H.B. is on the Institut National de la Recherche Agronomique staff. P.C. is an international research investigator from the Howard Hughes Medical Institute.
Funders:
Funding AgencyGrant Number
NIH Predoctoral FellowshipT32GM07104
NIHAI33987
European CommissionQLG2-CT-1999-00932
Ministère de l’Education Nationale et de la Recherche Scientifique et TechniqueUNSPECIFIED
Pasteur InstituteUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:4
DOI:10.1046/j.1365-2958.2002.02798.x
Record Number:CaltechAUTHORS:20200309-161404837
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200309-161404837
Official Citation:Bierne, H., Mazmanian, S.K., Trost, M., Pucciarelli, M.G., Liu, G., Dehoux, P., , Jänsch, L., Portillo, F.G.‐d., Schneewind, O. and Cossart, P. (2002), Inactivation of the srtA gene in Listeria monocytogenes inhibits anchoring of surface proteins and affects virulence. Molecular Microbiology, 43: 869-881. doi:10.1046/j.1365-2958.2002.02798.x
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:101799
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:10 Mar 2020 14:51
Last Modified:16 Nov 2021 18:06

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