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Passage of Heme-Iron Across the Envelope of Staphylococcus aureus

Mazmanian, Sarkis K. and Skaar, Eric P. and Gaspar, Andrew H. and Humayun, Munir and Gornicki, Piotr and Jelenska, Joanna and Joachmiak, Andrezj and Missiakas, Dominique M. and Schneewind, Olaf (2003) Passage of Heme-Iron Across the Envelope of Staphylococcus aureus. Science, 299 (5608). pp. 906-909. ISSN 0036-8075. https://resolver.caltech.edu/CaltechAUTHORS:20200309-161405106

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Abstract

The cell wall envelope of Gram-positive pathogens functions as a scaffold for the attachment of virulence factors and as a sieve that prevents diffusion of molecules. Here the isdgenes (iron-regulated surface determinant) of Staphylococcus aureus were found to encode factors responsible for hemoglobin binding and passage of heme-iron to the cytoplasm, where it acts as an essential nutrient. Heme-iron passage required two sortases that tether Isd proteins to unique locations within the cell wall. Thus, Isd appears to act as an import apparatus that uses cell wall–anchored proteins to relay heme-iron across the bacterial envelope.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1126/science.1081147DOIArticle
https://www.sciencemag.org/cgi/content/full/299/5608/906/DC1PublisherSupporting Information
ORCID:
AuthorORCID
Mazmanian, Sarkis K.0000-0003-2713-1513
Humayun, Munir0000-0002-5830-5208
Additional Information:© 2003 American Association for the Advancement of Science. 31 October 2002; accepted 7 January 2003. We thank D. E. Heinrichs (University of Western Ontario) for providing reagents. This work was supported by grants from the National Institutes of Health to D.M.M. (GM58266) and O.S. (AI38897 and AI5474).
Funders:
Funding AgencyGrant Number
NIHGM58266
NIHAI38897
NIHAI5474
Issue or Number:5608
Record Number:CaltechAUTHORS:20200309-161405106
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200309-161405106
Official Citation:Passage of Heme-Iron Across the Envelope of Staphylococcus aureus BY SARKIS K. MAZMANIAN, ERIC P. SKAAR, ANDREW H. GASPAR, MUNIR HUMAYUN, PIOTR GORNICKI, JOANNA JELENSKA, ANDRZEJ JOACHMIAK, DOMINIQUE M. MISSIAKAS, OLAF SCHNEEWIND Science 07 Feb 2003: Vol. 299, Issue 5608, pp. 906-909 DOI: 10.1126/science.1081147
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:101800
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:10 Mar 2020 14:48
Last Modified:10 Mar 2020 14:48

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