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Structures of Sortase B from Staphylococcus aureus and Bacillus anthracis Reveal Catalytic Amino Acid Triad in the Active Site

Zhang, Rongguang and Wu, Ruiying and Joachimiak, Grazyna and Mazmanian, Sarkis K. and Missiakas, Dominique M. and Gornicki, Piotr and Schneewind, Olaf and Joachimiak, Andrzej (2004) Structures of Sortase B from Staphylococcus aureus and Bacillus anthracis Reveal Catalytic Amino Acid Triad in the Active Site. Structure, 12 (7). pp. 1147-1156. ISSN 0969-2126. PMCID PMC2792001. https://resolver.caltech.edu/CaltechAUTHORS:20200309-161405803

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Abstract

Surface proteins attached by sortases to the cell wall envelope of bacterial pathogens play important roles during infection. Sorting and attachment of these proteins is directed by C-terminal signals. Sortase B of S. aureus recognizes a motif NPQTN, cleaves the polypeptide after the Thr residue, and attaches the protein to pentaglycine cross-bridges. Sortase B of B. anthracis is thought to recognize the NPKTG motif, and attaches surface proteins to m-diaminopimelic acid cross-bridges. We have determined crystal structure of sortase B from B. anthracis and S. aureus at 1.6 and 2.0 Å resolutions, respectively. These structures show a β-barrel fold with α-helical elements on its outside, a structure thus far exclusive to the sortase family. A putative active site located on the edge of the β-barrel is comprised of a Cys-His-Asp catalytic triad and presumably faces the bacterial cell surface. A putative binding site for the sorting signal is located nearby.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.str.2004.06.001DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2792001/PubMed CentralArticle
ORCID:
AuthorORCID
Mazmanian, Sarkis K.0000-0003-2713-1513
Additional Information:© 2004 Elsevier. Under an Elsevier user license. Received 29 April 2003, Revised 26 April 2004, Accepted 28 April 2004, Available online 13 July 2004. We thank all members of the SBC at ANL for their help conducting experiments and Lindy Keller for her help in preparation of this manuscript. This work was supported by grants from the National Institutes of Health, National Institute of General Medical Sciences to A.J. (GM62414), D.M.M. (GM58266), and O.S. (AI38897 and AI52474); by a University of Chicago-Argonne National Laboratory joint research grant under H.28 of the prime contract; by the U.S. Department of Energy, Office of Biological and Environmental Research, under contract W-31-109-Eng-38; and by the Regional Center of Excellence in Biodefense and Emerging Infectious Diseases (RCE) Program. The authors wish to acknowledge membership and support from the Region V (Great Lakes) RCE, award 1-U54-AI-057153 from the National Institutes of Allergy and Infectious Diseases. The submitted manuscript has been created by the University of Chicago as Operator of Argonne National Laboratory (“Argonne”) under Contract No. W-31-109-ENG-38 with the U.S. Department of Energy. The U.S. Government retains for itself, and others acting on its behalf, a paid-up, nonexclusive, irrevocable worldwide license in said article to reproduce, prepare derivative works, distribute copies to the public, and perform publicly and display publicly, by or on behalf of the Government. Accession Numbers: The atomic coordinates have been deposited in the Protein Data Bank, www.rcsb.org (PDB ID codes 1NG5 and 1RZ2).
Funders:
Funding AgencyGrant Number
NIHGM62414
NIHGM58266
NIHAI38897
NIHAI52474
University of Chicago-Argonne National Laboratory joint research grantUNSPECIFIED
Department of Energy (DOE)W-31-109-ENG-38
NIH1-U54-AI-057153
Issue or Number:7
PubMed Central ID:PMC2792001
Record Number:CaltechAUTHORS:20200309-161405803
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200309-161405803
Official Citation:Rongguang Zhang, Ruiying Wu, Grazyna Joachimiak, Sarkis K Mazmanian, Dominique M Missiakas, Piotr Gornicki, Olaf Schneewind, Andrzej Joachimiak, Structures of Sortase B from Staphylococcus aureus and Bacillus anthracis Reveal Catalytic Amino Acid Triad in the Active Site, Structure, Volume 12, Issue 7, 2004, Pages 1147-1156, ISSN 0969-2126, https://doi.org/10.1016/j.str.2004.06.001.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:101803
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:10 Mar 2020 14:39
Last Modified:10 Mar 2020 14:39

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