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Staphylococcus aureus Sortase, an Enzyme that Anchors Surface Proteins to the Cell Wall

Mazmanian, Sarkis K. and Liu, Gwen and Ton-That, Hung and Schneewind, Olaf (1999) Staphylococcus aureus Sortase, an Enzyme that Anchors Surface Proteins to the Cell Wall. Science, 285 (5428). pp. 760-763. ISSN 0036-8075. doi:10.1126/science.285.5428.760.

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Surface proteins of Gram-positive bacteria are linked to the bacterial cell wall by a mechanism that involves cleavage of a conserved Leu-Pro-X-Thr-Gly (LPXTG) motif and that occurs during assembly of the peptidoglycan cell wall. A Staphylococcus aureus mutant defective in the anchoring of surface proteins was isolated and shown to carry a mutation in the srtA gene. Overexpression of srtA increased the rate of surface protein anchoring, and homologs of srtA were found in other pathogenic Gram-positive bacteria. The protein specified bysrtA, sortase, may be a useful target for the development of new antimicrobial drugs.

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Mazmanian, Sarkis K.0000-0003-2713-1513
Additional Information:© 1999 American Association for the Advancement of Science. 6 May 1999; accepted 28 June 1999. We thank W. W. Navarre for help in determining the surface protein anchor structure of strain SM317, and D. Missiakas, P. Model, M. Russel, and members of our laboratory for critical reading of this manuscript. S.K.M. was supported by the Predoctoral Training Program in Genetic Mechanisms at UCLA (T32GM07104). H.T.-T. was supported by the Microbial Pathogenesis Training Grant at UCLA (AI 07323). Work in O.S.'s laboratory is supported by grant AI33987 from the National Institute of Allergy and Infectious Diseases.
Funding AgencyGrant Number
NIH Predoctoral FellowshipT32GM07104
NIH Predoctoral FellowshipAI07323
Issue or Number:5428
Record Number:CaltechAUTHORS:20200310-142734417
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Official Citation:Staphylococcus aureus Sortase, an Enzyme that Anchors Surface Proteins to the Cell Wall BY SARKIS K. MAZMANIAN, GWEN LIU, HUNG TON-THAT, OLAF SCHNEEWIND Science 30 Jul 1999: Vol. 285, Issue 5428, pp. 760-763 DOI: 10.1126/science.285.5428.760
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:101832
Deposited By: George Porter
Deposited On:10 Mar 2020 22:02
Last Modified:16 Nov 2021 18:06

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