Ashaber, Mária and Tomina, Yusuke and Kassraian, Pegah and Bushong, Eric A. and Kristan, William B., Jr. and Ellisman, Mark H. and Wagenaar, Daniel A. (2020) Anatomy and activity patterns in a multifunctional motor neuron and its surrounding circuits. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20200312-124530478
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Abstract
Dorsal Excitor motor neuron DE-3 in the medicinal leech plays three very different dynamical roles in three different behaviors. Without rewiring its anatomical connectivity, how can a motor neuron dynamically switch roles to play appropriate roles in various behaviors? We previously used voltage-sensitive dye imaging to record from DE-3 and most other neurons in the leech segmental ganglion during (fictive) swimming, crawling, and local-bend escape (Tomina and Wagenaar, 2017). Here, we repeated that experiment, then re-imaged the same ganglion using serial blockface electron microscopy and traced all of DE-3’s processes. Further, we traced back the processes of all of DE-3’s presynaptic partners to their respective somata. This allowed us to analyze the relationship between circuit anatomy and the activity patterns it sustains. We found that input synapses important for all of the behaviors were widely distributed over DE-3’s branches, yet that functional clusters were different during (fictive) swimming vs. crawling.
| Item Type: | Report or Paper (Discussion Paper) | |||||||||||||||||||||
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| Alternate Title: | Combined membrane potential imaging and connectome of behavioral circuits in an annelid worm, Imaging and connectome of leech ganglion | |||||||||||||||||||||
| Additional Information: | The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. Version 1 March 10, 2020; Version 2 August 7, 2020; Version 3 November 30, 2020. We thank Evan Miller (Berkeley) for sharing of the VF2.1(OMe).H dye, Art Wetzel (Pittsburgh Supercomputer Center) and Tom Bartol (The Salk Institute) for many useful discussions about image alignment, Tünde Magyar and Renáta Pop (University of Veterinary Medicine, Department of Anatomy and Histology, Budapest, Hungary) for help with TEM, and Jason Pipkin (Brandeis University) for advice about tracing. This work was supported by the National Institute of Neurological Disorders and Stroke (R01-NS094403 to DAW, MHE, and WBK) and by the National Institute of General Medical Sciences (P41GM103412 in support of the National Center for Molecular Imaging Research to MHE). YT was supported by Japan Society for Promotion of Science (Overseas Research Fellowship, Research Fellowship for Young Scientists PD, 201800526 and Grant-in-Aid for Young Scientists, 19K16191). PK was supported by the Swiss National Science Foundation (P2EZP3-181896). Author contributions: Conceptualization: YT, PK, MHE,WBK, DAW. Data curation: YT, DAW. Formal analysis: YT, MA, PK, DAW. Funding acquisition: YT, PK, MHE, WBK, DAW. Investigation: YT, MA, PK, EAB. Methodology: All. Software: PK, DAW. Supervision: MHE, DAW. Visualization: YT, MA, PK, DAW. Writing—original draft: YT, MA. Writing—review and editing: YT, MA, PK, DAW. The authors declare no competing interests. Data and materials availability: The easiest way to access the raw electrophysiology and voltage-dye data as well as the tracing results used in this paper is through a series of Python modules that we made available at https://github.com/wagenadl/leechem-public. Included in the package is a file called “demo.py” that demonstrates the use of the modules. Supplemental table S4 lists the available VSD trials. The aligned EM volume may be accessed through the Neuroglancer (Google, 2016) instance at https://leechem.caltech.edu or by pointing SBEMViewer to https://leechem.caltech.edu/emdata. The code used for alignment is available at https://github.com/wagenadl/sbemalign. Our visualization tools SBEMViewer and GVox are at https://github.com/wagenadl/sbemviewer and https://github.com/wagenadl/gvox. | |||||||||||||||||||||
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| Record Number: | CaltechAUTHORS:20200312-124530478 | |||||||||||||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20200312-124530478 | |||||||||||||||||||||
| Official Citation: | Anatomy and activity patterns in a multifunctional motor neuron and its surrounding circuits Mária Ashaber, Yusuke Tomina, Pegah Kassraian, Eric A. Bushong, William B. Kristan Jr, Mark H. Ellisman, Daniel A. Wagenaar bioRxiv 2020.03.09.984013; doi: https://doi.org/10.1101/2020.03.09.984013 | |||||||||||||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||||||||||||||
| ID Code: | 101894 | |||||||||||||||||||||
| Collection: | CaltechAUTHORS | |||||||||||||||||||||
| Deposited By: | Tony Diaz | |||||||||||||||||||||
| Deposited On: | 12 Mar 2020 20:12 | |||||||||||||||||||||
| Last Modified: | 03 Dec 2020 22:25 |
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