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Structures of Natively-Glycosylated HIV-1 Envelope Trimers Define Antibody-Mediated Neutralization of HIV-1

Barnes, Christopher O. (2020) Structures of Natively-Glycosylated HIV-1 Envelope Trimers Define Antibody-Mediated Neutralization of HIV-1. Biophysical Journal, 118 (3). 3a. ISSN 0006-3495. https://resolver.caltech.edu/CaltechAUTHORS:20200319-084323389

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Abstract

Broadly neutralizing antibodies (bNAbs) isolated from HIV-1 infected individuals inform vaccine design and are potential therapeutic agents. However, developing bNAbs with increased efficacy requires understanding how antibodies interact with the native oligomannose and complex-type N-glycan shield that hides most protein epitopes on HIV-1 envelope (Env). We present single-particle cryoEM structures of natively-glycosylated Env trimers complexed with newly identified bNAbs that target distinct epitopes on the surface of Env. A 3.3Å cryoEM structure of Env bound to SF12, an antibody that recognizes a glycan-dominated epitope on Env's silent face, revealed distinct N-glycan conformations across Env protomers, demonstrating intra-Env glycan heterogeneity. Insights from these antibody-Env complex structures have facilitated our understanding of bNAb-glycan interactions critical for their potential use in HIV-1 prevention, therapy, and vaccine development.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.bpj.2019.11.211DOIArticle
ORCID:
AuthorORCID
Barnes, Christopher O.0000-0003-2754-5951
Additional Information:© 2020 Biophysical Society. Available online 7 February 2020.
Issue or Number:3
Record Number:CaltechAUTHORS:20200319-084323389
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200319-084323389
Official Citation:Christopher O. Barnes, Structures of Natively-Glycosylated HIV-1 Envelope Trimers Define Antibody-Mediated Neutralization of HIV-1, Biophysical Journal, Volume 118, Issue 3, Supplement 1, 2020, Page 3a, ISSN 0006-3495, https://doi.org/10.1016/j.bpj.2019.11.211. (http://www.sciencedirect.com/science/article/pii/S0006349519311403)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:101989
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:19 Mar 2020 16:10
Last Modified:03 Aug 2020 22:13

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