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Fluorescence imaging through dynamic scattering media with speckle-encoded ultrasound-modulated light correlation

Ruan, Haowen and Liu, Yan and Xu, Jian and Huang, Yujia and Yang, Changhuei (2020) Fluorescence imaging through dynamic scattering media with speckle-encoded ultrasound-modulated light correlation. Nature Photonics, 14 (8). pp. 511-516. ISSN 1749-4885. https://resolver.caltech.edu/CaltechAUTHORS:20200324-095704695

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Abstract

Fluorescence imaging is indispensable to biomedical research, and yet it remains challenging to image through dynamic scattering samples. Techniques that combine ultrasound and light as exemplified by ultrasound-assisted wavefront shaping have enabled fluorescence imaging through scattering media. However, the translation of these techniques into in vivo applications has been hindered by the lack of high-speed solutions to counter the fast speckle decorrelation of dynamic tissue. Here, we report an ultrasound-enabled optical imaging method that instead leverages the dynamic nature to perform imaging. The method utilizes the correlation between the dynamic speckle-encoded fluorescence and ultrasound-modulated light signal that originate from the same location within a sample. We image fluorescent targets with an improved resolution of ≤75 µm (versus a resolution of 1.3 mm with direct optical imaging) within a scattering medium with 17 ms decorrelation time. This new imaging modality paves the way for fluorescence imaging in highly scattering tissue in vivo.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1038/s41566-020-0630-0DOIArticle
https://rdcu.be/b353SPublisherFree ReadCube access
ORCID:
AuthorORCID
Ruan, Haowen0000-0002-4917-4509
Liu, Yan0000-0002-5837-4908
Xu, Jian0000-0002-4743-2471
Huang, Yujia0000-0001-7667-8342
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2020 Springer Nature Limited. Received 28 January 2020; Accepted 30 March 2020; Published 11 May 2020. This work was supported by the Kernel–Brain Research and Technologies fund (FS 13520230) and the Rosen Bioengineering Center Endowment Fund (9900050). Data availability: The data that support the plots within this paper and other findings of this study are available from the corresponding authors upon reasonable request. Code availability: The code that supports the plots within this paper and other findings of this study is available from the corresponding authors upon reasonable request. Author Contributions: H.R. conceived the idea. H.R., Y.L. and C.Y. developed the idea and designed the experiments. Y.L. and H.R. developed the experimental protocol and set-up. Y.L. constructed the samples and conducted the imaging experiments. H.R. and Y.L. analysed the data. C.Y., J.X., H.R., Y.L. and Y.H. conducted the theoretical analysis. All authors contributed to the preparation of the manuscript. The authors declare no competing interests.
Group:Rosen Bioengineering Center
Funders:
Funding AgencyGrant Number
Kernel-Brain Research and TechnologiesFS 13520230
Donna and Benjamin M. Rosen Bioengineering Center9900050
Subject Keywords:Adaptive optics; Biophotonics; Fluorescence imaging; Imaging and sensing
Issue or Number:8
Record Number:CaltechAUTHORS:20200324-095704695
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200324-095704695
Official Citation:Ruan, H., Liu, Y., Xu, J. et al. Fluorescence imaging through dynamic scattering media with speckle-encoded ultrasound-modulated light correlation. Nat. Photonics 14, 511–516 (2020). https://doi.org/10.1038/s41566-020-0630-0
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102075
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:27 Apr 2020 18:30
Last Modified:28 Jul 2020 20:19

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