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Effects of antibodies against dynein and tubulin on the stiffness of flagellar axonemes

Okuno, M. and Asai, D. J. and Ogawa, K. and Brokaw, C. J. (1981) Effects of antibodies against dynein and tubulin on the stiffness of flagellar axonemes. Journal of Cell Biology, 91 (3). pp. 689-694. ISSN 0021-9525. PMCID PMC2112789. https://resolver.caltech.edu/CaltechAUTHORS:OKUjcb81

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Abstract

Antidynein antibodies, previously shown to inhibit flagellar oscillation and active sliding of axonemal microtubules, increase the bending resistance of axonemes measured under relaxing conditions, but not the bending resistance of axonemes measured under rigor conditions. These observations suggest that antidynein antibodies can stabilize rigor cross-bridges between outer-doublet microtubules, by interfering with ATP-induced cross-bridge detachment. Stabilization of a small number of cross-bridge appears to be sufficient to cause substantial inhibition of the frequency of flagellar oscillation. Antitubulin antibodies, previously shown to inhibit flagellar oscillation without inhibiting active sliding of axonemal microtubules, do not increase the static bending resistance of axonemes. However, we observed a viscoelastic effect, corresponding to a large increase in the immediate bending resistance. This immediate bending resistance increase may be sufficient to explain inhibition of flagellar oscillation; but several alternative explanations cannot yet be excluded.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1083/jcb.91.3.689DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc2112789/PubMed CentralArticle
Additional Information:© 1981 by The Rockefeller University Press. Received for publication 15 June 1981. We thank T.F. Simonick for assistance with these experiments. This work was supported by National Institutes of Health grant GM-18711 from the National Institute of General Medical Sciences.
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Funding AgencyGrant Number
NIHGM-18711
Issue or Number:3
PubMed Central ID:PMC2112789
Record Number:CaltechAUTHORS:OKUjcb81
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:OKUjcb81
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ID Code:10235
Collection:CaltechAUTHORS
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Deposited On:17 Apr 2008
Last Modified:08 Jul 2020 15:49

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