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A Fence-like Coat for the Nuclear Pore Membrane

Debler, Erik W. and Ma, Yingli and Seo, Hyuk-Soo and Hsia, Kuo-Chiang and Noriega, Thomas R. and Blobel, Günter and Hoelz, André (2008) A Fence-like Coat for the Nuclear Pore Membrane. Molecular Cell, 32 (6). pp. 815-826. ISSN 1097-2765. doi:10.1016/j.molcel.2008.12.001.

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We recently proposed a cylindrical coat for the nuclear pore membrane in the nuclear pore complex (NPC). This scaffold is generated by multiple copies of seven nucleoporins. Here, we report three crystal structures of the nucleoporin pair Seh1•Nup85, which is part of the coat cylinder. The Seh1•Nup85 assembly bears resemblance in its shape and dimensions to that of another nucleoporin pair, Sec13•Nup145C. Furthermore, the Seh1•Nup85 structures reveal a hinge motion that may facilitate conformational changes in the NPC during import of integral membrane proteins and/or during nucleocytoplasmic transport. We propose that Seh1•Nup85 and Sec13•Nup145C form 16 alternating, vertical rods that are horizontally linked by the three remaining nucleoporins of the coat cylinder. Shared architectural and mechanistic principles with the COPII coat indicate a common evolutionary origin and support the notion that the NPC coat represents another class of membrane coats.

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Hoelz, André0000-0003-1726-0127
Additional Information:© 2008 Elsevier Inc. Under an Elsevier user license. Received 8 September 2008, Revised 21 November 2008, Accepted 2 December 2008, Available online 24 December 2008. We thank O. Dreesen, I. Melčák, V. Nagy, J. Napetschnig, A. Patke, and P. Stavropoulos for discussions and comments on the manuscript; S. Etherton for help with editing the manuscript; D. King for mass spectrometry analysis; P. Stavropoulos and E. Hurt for providing material; and J. Champagne for assistance with the light scattering analysis. In addition, we thank C. Ralston (ALS), M. Becker and R. Fischetti (GM/CA-CAT), K. Rajashankar and N. Sukumar (NE-CAT), and W. Shi (NSLS) for support during data collection. N-terminal protein sequencing was performed by the Protein Center of the Rockefeller University; analytical ultracentrifugation was carried out by the Wadsworth Center Biochemistry Core Facility. E.W.D. is the Dale F. and Betty Ann Frey Fellow of the Damon Runyon Cancer Research Foundation, DRG-1977-08. A.H. was supported by a grant from the Leukemia and Lymphoma Society. Accession Numbers: The atomic coordinates and structure factors of the three crystal structures have been deposited to the Protein Data Bank with the accession codes 3F3F, 3F3G, and 3F3P.
Funding AgencyGrant Number
Damon Runyon Cancer Research FoundationDRG-1977-08
Leukemia and Lymphoma SocietyUNSPECIFIED
Subject Keywords:PROTEINS; CELLBIO
Issue or Number:6
Record Number:CaltechAUTHORS:20200407-094405550
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Official Citation:Erik W. Debler, Yingli Ma, Hyuk-Soo Seo, Kuo-Chiang Hsia, Thomas R. Noriega, Günter Blobel, André Hoelz, A Fence-like Coat for the Nuclear Pore Membrane, Molecular Cell, Volume 32, Issue 6, 2008, Pages 815-826, ISSN 1097-2765, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102376
Deposited By: Tony Diaz
Deposited On:07 Apr 2020 16:58
Last Modified:16 Nov 2021 18:11

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