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Architecture of a Coat for the Nuclear Pore Membrane

Hsia, Kuo-Chiang and Stavropoulos, Pete and Blobel, Günter and Hoelz, André (2007) Architecture of a Coat for the Nuclear Pore Membrane. Cell, 131 (7). pp. 1313-1326. ISSN 0092-8674. PMCID PMC2213454. doi:10.1016/j.cell.2007.11.038. https://resolver.caltech.edu/CaltechAUTHORS:20200407-100337903

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Abstract

The symmetric core of the nuclear pore complex can be considered schematically as a series of concentric cylinders. A peripheral cylinder coating the pore membrane contains the previously characterized, elongated heptamer that harbors Sec13-Nup145C in its middle section. Strikingly, Sec13-Nup145C crystallizes as a hetero-octamer in two space groups. Oligomerization of Sec13-Nup145C was confirmed biochemically. Importantly, the numerous interacting surfaces in the hetero-octamer are evolutionarily highly conserved, further underlining the physiological relevance of the oligomerization. The hetero-octamer forms a slightly curved, yet rigid rod of sufficient length to span the entire height of the proposed membrane-adjacent cylinder. In concordance with the dimensions and symmetry of the nuclear pore complex core, we suggest that the cylinder is constructed of four antiparallel rings, each ring being composed of eight heptamers arranged in a head-to-tail fashion. Our model proposes that the hetero-octamer would vertically traverse and connect the four stacked rings.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.cell.2007.11.038DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213454PubMed CentralArticle
ORCID:
AuthorORCID
Hoelz, André0000-0003-1726-0127
Additional Information:© 2007 Elsevier Inc. Received 27 August 2007, Revised 26 October 2007, Accepted 29 November 2007, Available online 27 December 2007. We thank E. Debler, O. Dreesen, I. Melcak, V. Nagy, J. Napetschnig, and A. Patke for discussions and comments on the manuscript, S. Etherton for help with editing the manuscript, and D. King for mass spectrometry analysis. In addition, we thank C. Ralston, B. Manjasetty, and W. Shi for support during data collection. N-terminal protein sequencing was performed by the Protein Center of the Rockefeller University. A.H. was supported by a grant from the Leukemia and Lymphoma Society. Accession Numbers: The atomic coordinates of the monoclinic and orthorhombic structures have been deposited to the Protein Data Bank with the accession codes 3BG0 and 3BG1.
Funders:
Funding AgencyGrant Number
Leukemia and Lymphoma SocietyUNSPECIFIED
Subject Keywords:CELLBIO; SIGNALING
Issue or Number:7
PubMed Central ID:PMC2213454
DOI:10.1016/j.cell.2007.11.038
Record Number:CaltechAUTHORS:20200407-100337903
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200407-100337903
Official Citation:Kuo-Chiang Hsia, Pete Stavropoulos, Günter Blobel, André Hoelz, Architecture of a Coat for the Nuclear Pore Membrane, Cell, Volume 131, Issue 7, 2007, Pages 1313-1326, ISSN 0092-8674, https://doi.org/10.1016/j.cell.2007.11.038. (http://www.sciencedirect.com/science/article/pii/S0092867407015425)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102378
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:07 Apr 2020 17:45
Last Modified:16 Nov 2021 18:11

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