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Oligomerization states of the association domain and the holoenyzme of Ca²⁺/CaM kinase II

Rosenberg, Oren S. and Deindl, Sebastian and Comolli, Luis R. and Hoelz, André and Downing, Kenneth H. and Nairn, Angus C. and Kuriyan, John (2006) Oligomerization states of the association domain and the holoenyzme of Ca²⁺/CaM kinase II. FEBS Journal, 273 (4). pp. 682-694. ISSN 1742-464X. https://resolver.caltech.edu/CaltechAUTHORS:20200407-122406569

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Abstract

Ca²⁺/calmodulin activated protein kinase II (CaMKII) is an oligomeric protein kinase with a unique holoenyzme architecture. The subunits of CaMKII are bound together into the holoenzyme by the association domain, a C‐terminal region of ≈ 140 residues in the CaMKII polypeptide. Single particle analyses of electron micrographs have suggested previously that the holoenyzme forms a dodecamer that contains two stacked 6‐fold symmetric rings. In contrast, a recent crystal structure of the isolated association domain of mouse CaMKIIα has revealed a tetradecameric assembly with two stacked 7‐fold symmetric rings. In this study, we have determined the crystal structure of the Caenorhabditis elegans CaMKII association domain and it too forms a tetradecamer. We also show by electron microscopy that in its fully assembled form the CaMKII holoenzyme is a dodecamer but without the kinase domains, either from expression of the isolated association domain in bacteria or following their removal by proteolysis, the association domains form a tetradecamer. We speculate that the holoenzyme is held in its 6‐fold symmetric state by the interactions of the N‐terminal ≈ 1–335 residues and that the removal of this region allows the association domain to convert into a more stable 7‐fold symmetric form.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1111/j.1742-4658.2005.05088.xDOIArticle
ORCID:
AuthorORCID
Hoelz, André0000-0003-1726-0127
Additional Information:© 2006 The Authors. Journal compilation © 2006 FEBS. Received 6 September 2005, revised 27 November 2005, accepted 5 December 2005. We thank Robert Glaeser, Pietro de Camilli, Karin Reinisch and Cori Bargmann for helpful discussions. We thank Xiaoxian Cao, Holger Sondermann, Cori Ralston, David King and Arnie Falick for invaluable technical expertise. The cDNA for UNC‐43 splice variant K11E8.d was a kind gift of Dr Min Han. Oren Rosenberg is supported by the Yale Medical Scientist Training Grant. Parts of this work have been supported by the Director, Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy under Contract No. DE‐AC02‐05CH11231.
Funders:
Funding AgencyGrant Number
Yale UniversityUNSPECIFIED
Department of Energy (DOE)DE‐AC02‐05CH11231
Subject Keywords:association domain; Ca2+/calmodulin dependent protein; kinase II; holoenzyme; kinase activation; oligomerization
Issue or Number:4
Record Number:CaltechAUTHORS:20200407-122406569
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200407-122406569
Official Citation:Rosenberg, O.S., Deindl, S., Comolli, L.R., Hoelz, A., Downing, K.H., Nairn, A.C. and Kuriyan, J. (2006), Oligomerization states of the association domain and the holoenyzme of Ca2+/CaM kinase II. The FEBS Journal, 273: 682-694. doi:10.1111/j.1742-4658.2005.05088.x
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102384
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:07 Apr 2020 19:36
Last Modified:07 Apr 2020 19:36

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