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Nanoneedle Platforms: The Many Ways to Pierce the Cell Membrane

He, Gen and Hu, Ning and Xu, Alexander M. and Li, Xiangling and Zhao, Yunlong and Xie, Xi (2020) Nanoneedle Platforms: The Many Ways to Pierce the Cell Membrane. Advanced Functional Materials, 30 (21). Art. No. 1909890. ISSN 1616-301X. https://resolver.caltech.edu/CaltechAUTHORS:20200408-125209697

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Abstract

Establishing techniques to efficiently and nondestructively access the intracellular milieu is essential for many biomedical and scientific applications, ranging from drug delivery, to electrical recording, to biochemical detection. Cell penetration using nanoneedle arrays is currently a research focus area because it not only meets the increasing therapeutic demands of cell modifications and genome editing, but also provides an ideal platform for tracking long‐term intracellular information. Although the precise mechanism driving membrane penetration by nanoneedle arrays is still unclear, the low cytotoxicity, wide range of delivered materials, diverse cell type targets, and simple material structures of nanoneedle arrays make these splendid platforms for cell access. Here, the recent progress in this field is reviewed by examining device architectures and discussing mechanisms for nanoneedle penetration, and the major studies demonstrating the most general applicability of nanoneedle arrays, typical methodologies to access the intracellular environment using nanoneedles with spontaneous or assisted penetration modes, as well as biosafety aspects are presented. This review should be valuable for deeply understanding the materials fabrication principles, device designs, cell penetration methodologies, biosafety aspects, and application strategies of nanoneedle array‐based systems that are of crucial importance for the development of future practical biomedical platforms.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/adfm.201909890DOIArticle
ORCID:
AuthorORCID
Xu, Alexander M.0000-0003-4877-4358
Xie, Xi0000-0001-7406-8444
Additional Information:© 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Received: November 27, 2019; Revised: January 20, 2020; Published online: March 4, 2020. G.H., N.H., and A.M.X. contributed equally to this work. The authors would like to acknowledge financial support from the National Natural Science Foundation of China (Grant Nos. 61771498, 61901535, 51805556, and 31900954), the Science and Technology Planning Project of Guangdong Province for Industrial Applications (Grant No. 2017B090917001), Guangdong Province Key Area R&D Program (Grant No.2018B030332001), and the Science and Technology Program of Guangzhou, China (Grant No. 201907010038). The authors wish to thank Prof. Nicholas A. Melosh at Stanford University for his careful guidance and suggestion on the discussion of the penetration mechanism. The authors declare no conflict of interest.
Funders:
Funding AgencyGrant Number
National Natural Science Foundation of China61771498
National Natural Science Foundation of China61901535
National Natural Science Foundation of China51805556
National Natural Science Foundation of China31900954
Science and Technology Planning Project of Guangdong Province for Industrial Applications2017B090917001
Guangdong Province Key Area R&D Program2018B030332001
Science and Technology Program of Guangzhou201907010038
Subject Keywords:assisted penetration, cell biosafety, intracellular applications, nanoneedle arrays, spontaneous penetration
Issue or Number:21
Record Number:CaltechAUTHORS:20200408-125209697
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200408-125209697
Official Citation:He, G., Hu, N., Xu, A. M., Li, X. L., Zhao, Y. L., Xie, X., Nanoneedle Platforms: The Many Ways to Pierce the Cell Membrane. Adv. Funct. Mater. 2020, 30, 1909890. https://doi.org/10.1002/adfm.201909890
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102401
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 Apr 2020 21:04
Last Modified:21 May 2020 21:33

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