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Regulation of the formin cappuccino is critical for polarity of Drosophilao ocytes

Bor, Batbileg and Bois, Justin S. and Quinlan, Margot E. (2015) Regulation of the formin cappuccino is critical for polarity of Drosophilao ocytes. Cytoskeleton, 72 (1). pp. 1-15. ISSN 1949-3584. PMCID PMC4361322. https://resolver.caltech.edu/CaltechAUTHORS:20200413-082218419

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Abstract

The Drosophila formin Cappuccino (Capu) creates an actin mesh‐like structure that traverses the oocyte during midoogenesis. This mesh is thought to prevent premature onset of fast cytoplasmic streaming which normally happens during late‐oogenesis. Proper cytoskeletal organization and cytoplasmic streaming are crucial for localization of polarity determinants such as osk, grk, bcd, and nanos mRNAs. Capu mutants disrupt these events, leading to female sterility. Capu is regulated by another nucleator, Spire, as well as by autoinhibition in vitro. Studies in vivo confirm that Spire modulates Capu's function in oocytes; however, how autoinhibition contributes is still unclear. To study the role of autoinhibition in flies, we expressed a Capu construct that is missing the Capu Inhibitory Domain, CapuΔN. Consistent with a gain of activity due to loss of autoinhibition, the actin mesh was denser in CapuΔN oocytes. Further, cytoplasmic streaming was delayed and fertility levels decreased. Localization of osk mRNA in early stages, and bcd and nanos in late stages, were disrupted in CapuΔN‐expressing oocytes. Finally, evidence that these phenotypes were due to a loss of autoinhibition comes from coexpression of the N‐terminal half of Capu with CapuΔN, which suppressed the defects in actin, cytoplasmic streaming and fertility. From these results, we conclude that Capu can be autoinhibited during Drosophila oocyte development.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/cm.21205DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4361322PubMed CentralArticle
ORCID:
AuthorORCID
Bois, Justin S.0000-0001-7137-8746
Additional Information:© 2014 Wiley Periodicals, Inc. We thank the Gavis, Schüpbach and St. Johnston labs for generously sharing fly lines used in our experiments. We thank the De Robertis lab for use of their dark field microscope and the Courey lab for reagents and help with methods. B.B. thanks his parents (Bor Batsuuri and Altanchimeg Mishig) for bringing him to this world and to this country. This work was supported by several funding sources: UCLA Whitcome fellowship (B.B.); National Institutes of Health (R01GM096133), Burroughs-Welcome Fund (Career Award in the Biomedical Sciences), and March of Dimes Foundation (#1-FY12-442) (M.E.Q.). Stocks obtained from the Bloomington Drosophila Stock Center (NIH P4OD018537) were used in this study. The authors declare no competing financial interests.
Funders:
Funding AgencyGrant Number
UCLAUNSPECIFIED
NIHR01GM096133
Burroughs Wellcome FundUNSPECIFIED
March of Dimes Foundation1-FY12-442
NIHP4OD018537
Non-Subject Keywords:Capu; actin; mRNA; autoinhibition; polarity
Issue or Number:1
PubMed Central ID:PMC4361322
Record Number:CaltechAUTHORS:20200413-082218419
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200413-082218419
Official Citation:Bor, B., Bois, J.S. and Quinlan, M.E. (2015), Regulation of the formin cappuccino is critical for polarity of Drosophila oocytes. Cytoskeleton, 72: 1-15. doi:10.1002/cm.21205
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102492
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:13 Apr 2020 16:44
Last Modified:13 Apr 2020 16:44

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