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Egfr/Ras signaling regulates DE-cadherin/Shotgun localization to control vein morphogenesis in the Drosophila wing

O'Keefe, David D. and Prober, David A. and Moyle, Patrick S. and Rickoll, Wayne L. and Edgar, Bruce A. (2007) Egfr/Ras signaling regulates DE-cadherin/Shotgun localization to control vein morphogenesis in the Drosophila wing. Developmental Biology, 311 (1). pp. 25-39. ISSN 0012-1606. PMCID PMC2128780. doi:10.1016/j.ydbio.2007.08.003.

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Egfr/Ras signaling promotes vein cell fate specification in the developing Drosophila wing. While the importance of Ras signaling in vein determination has been extensively documented, the mechanisms linking Ras activity to vein differentiation remain unclear. We found that Ras signaling regulates both the levels and subcellular localization of the cell adhesion molecule DE-cadherin/Shotgun (Shg) in the differentiating wing epithelium. High Ras activity in presumptive vein cells directs the apical localization of Shg containing adherens junctions, whereas low Ras activity in intervein cells allows Shg to relocalize basally. These alterations in Shg-mediated adhesion control cell shape changes that are essential for vein morphogenesis. While Decapentaplegic (Dpp) acts downstream of Ras to maintain vein cell identity in the pupal wing, our results indicate that Ras controls Shg localization via a Dpp-independent mechanism. Ras, therefore, regulates both the transcriptional responses necessary for vein cell identity, and the cell adhesive changes that determine vein and intervein cell morphology.

Item Type:Article
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URLURL TypeDescription CentralArticle
Prober, David A.0000-0002-7371-4675
Additional Information:© 2007 Elsevier Inc. Received 13 March 2007, Revised 1 August 2007, Accepted 2 August 2007, Available online 9 August 2007. We would like to thank Seth Blair, Pernille Rorth, Ernst Hafen, Jose de Celis, Marta Llimargas, Celeste Berg, Hannele Ruohola-Baker, The Developmental Studies Hybridoma Bank, and the Bloomington Drosophila Stock Center for reagents. We are particularly grateful to Ryu Ueda and his colleagues at the National Institute of Genetics in Japan for generation of the UAS-RNAi transgenes used in this study. Thanks to the University of Puget Sound and Dr. Scott Weatherwax for supporting the UPS Weatherwax Electron microscopy Lab. Thanks to Julio Vazquez, Dave McDonald, and Adrian Quintanilla for help with confocal microscopy. Finally, thanks to Leslie Saucedo, Steve “Jahman” Lee, and the entire Edgar lab for advice and entertainment. This work was supported by NIH U56 CA096288 to Beti Thompson, NIH R01 GM51186 to B.A.E., and an American Cancer Society Postdoctoral Fellowship to D.D.O.
Funding AgencyGrant Number
NIHU56 CA096288
NIHR01 GM51186
American Cancer SocietyUNSPECIFIED
Subject Keywords:Drosophila; Cell adhesion; Adherens junction; Wing development; Wing vein; E-cadherin; Ras; Egfr
Issue or Number:1
PubMed Central ID:PMC2128780
Record Number:CaltechAUTHORS:20200415-122447337
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Official Citation:David D. O'Keefe, David A. Prober, Patrick S. Moyle, Wayne L. Rickoll, Bruce A. Edgar, Egfr/Ras signaling regulates DE-cadherin/Shotgun localization to control vein morphogenesis in the Drosophila wing, Developmental Biology, Volume 311, Issue 1, 2007, Pages 25-39, ISSN 0012-1606, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102560
Deposited By: Tony Diaz
Deposited On:15 Apr 2020 19:50
Last Modified:16 Nov 2021 18:13

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