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Caging proteins through unnatural amino acids mutagenesis

Petersson, E. James and Brandt, Gabriel S. and Zacharias, Niki M. and Dougherty, Dennis A. and Lester, Henry A. (2003) Caging proteins through unnatural amino acids mutagenesis. In: Biophotonics, Part A. Methods in Enzymology. No.360. Academic Press , San Diego, CA, pp. 258-273. ISBN 9780121822637.

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The caging of specific residues of proteins is a powerful tool. This discussion attempts to alert the reader to the considerations that must be made in preparing and analyzing a caged protein through nonsense suppression. Although the suppression methodology is conceptually straightforward, it not possible to provide a failsafe “cook book” method for using caged unnaturals. We have emphasized the preparation of caged receptors expressed in Xenopus oocytes, but these approaches can clearly be adapted to many other systems.

Item Type:Book Section
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URLURL TypeDescription
Petersson, E. James0000-0003-3854-9210
Dougherty, Dennis A.0000-0003-1464-2461
Lester, Henry A.0000-0002-5470-5255
Additional Information:© 2003 Published by Elsevier. This work was supported by the National Institutes of Health (Grants GM-29836, NS-11756, NS-34407, and NS-11756).
Funding AgencyGrant Number
Series Name:Methods in Enzymology
Issue or Number:360
Record Number:CaltechAUTHORS:20200417-142717296
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Official Citation:E. James Petersson, Gabriel S. Brandt, Niki M. Zacharias, Dennis A. Dougherty, Henry A. Lester, [10] Caging proteins through unnatural amino acids mutagenesis, Methods in Enzymology, Academic Press, Volume 360, 2003, Pages 258-273, ISSN 0076-6879, ISBN 9780121822637,
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102625
Deposited By: George Porter
Deposited On:20 Apr 2020 14:26
Last Modified:16 Nov 2021 18:13

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