Chen, Chun-Hong and Guo, Ming and Hay, Bruce A. (2006) Identifying MicroRNA Regulators of Cell Death in Drosophila. In: MicroRNA Protocols. Methods in Molecular Biology. No.342. Humana Press , Totowa, NJ, pp. 229-240. ISBN 978-1-58829-581-1. https://resolver.caltech.edu/CaltechAUTHORS:20200421-075723029
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Abstract
Animal genomes contain on the order of at least hundreds of microRNAs (miRNAs). Although most remain uncharacterized, it is already clear that miRNAs regulate many bio-logical processes. A number of Drosophila miRNAs have been identified as likely cell death regulators, but functions for most have simply not been explored. Here we describe a protocol for identifying miRNAs that can act as cell death regulators. We also describe a simple protocol for testing roles for mRNAs identified as candidate miRNA targets using computational or other approaches.
Item Type: | Book Section | ||||||
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Additional Information: | © 2006 Humana Press Inc. | ||||||
Subject Keywords: | Drosophila; apoptosis; caspase; microRNA; RNAi; cancer | ||||||
Series Name: | Methods in Molecular Biology | ||||||
Issue or Number: | 342 | ||||||
DOI: | 10.1385/1-59745-123-1:229 | ||||||
Record Number: | CaltechAUTHORS:20200421-075723029 | ||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20200421-075723029 | ||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
ID Code: | 102681 | ||||||
Collection: | CaltechAUTHORS | ||||||
Deposited By: | Tony Diaz | ||||||
Deposited On: | 21 Apr 2020 16:31 | ||||||
Last Modified: | 16 Nov 2021 18:14 |
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