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Histone acetylation increases chromatin accessibility

Görisch, Sabine M. and Wachsmuth, Malte and Fejes Tóth, Katalin and Lichter, Peter and Rippe, Karsten (2005) Histone acetylation increases chromatin accessibility. Journal of Cell Science, 118 (24). pp. 5825-5834. ISSN 0021-9533. https://resolver.caltech.edu/CaltechAUTHORS:20200421-085232522

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Abstract

In eukaryotes, the interaction of DNA with proteins and supramolecular complexes involved in gene expression is controlled by the dynamic organization of chromatin inasmuch as it defines the DNA accessibility. Here, the nuclear distribution of microinjected fluorescein-labeled dextrans of 42 kDa to 2.5 MDa molecular mass was used to characterize the chromatin accessibility in dependence on histone acetylation. Measurements of the fluorescein-dextran sizes were combined with an image correlation spectroscopy analysis, and three different interphase chromatin condensation states with apparent pore sizes of 16-20 nm, 36-56 nm and 60-100 nm were identified. A reversible change of the chromatin conformation to a uniform 60-100 nm pore size distribution was observed upon increased histone acetylation. This result identifies histone acetylation as a central factor in the dynamic regulation of chromatin accessibility during interphase. In mitotic chromosomes, the chromatin exclusion limit was 10-20 nm and independent of the histone acetylation state.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1242/jcs.02689DOIArticle
ORCID:
AuthorORCID
Fejes Tóth, Katalin0000-0001-6558-2636
Additional Information:© 2005 Company of Biologists. Accepted 12 September 2005. We are grateful to Cristina Cardoso, Stephanie Geiger, Stephanie Fesser and Thibaud Jegou for valuable help, and thank Marion Cremer and Roman Zinner for discussion. This work was supported by the Deutsche Forschungsgemeinschaft (grants Li406/5-3 and Ri828/5-1) and the Volkswagen Foundation in the program Junior Research Groups at German Universities.
Funders:
Funding AgencyGrant Number
Deutsche Forschungsgemeinschaft (DFG)Li406/5-3
Deutsche Forschungsgemeinschaft (DFG)Ri828/5-1
Volkswagen FoundationUNSPECIFIED
Subject Keywords:Heterochromatin; Image correlation spectroscopy; Trichostatin A; Microinjection
Issue or Number:24
Record Number:CaltechAUTHORS:20200421-085232522
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200421-085232522
Official Citation:Histone acetylation increases chromatin accessibility. Sabine M. Görisch, Malte Wachsmuth, Katalin Fejes Tóth, Peter Lichter, Karsten Rippe. Journal of Cell Science 2005 118: 5825-5834; doi: 10.1242/jcs.02689
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102688
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:21 Apr 2020 16:15
Last Modified:21 Apr 2020 16:15

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