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Trichostatin A-induced histone acetylation causes decondensation of interphase chromatin

Fejes Tóth, Katalin and Knoch, Tobias A. and Wachsmuth, Malte and Frank-Stöhr, Monika and Stöhr, Michael and Bacher, Christian P. and Müller, Gabriele and Rippe, Karsten (2004) Trichostatin A-induced histone acetylation causes decondensation of interphase chromatin. Journal of Cell Science, 117 (18). pp. 4277-4287. ISSN 0021-9533.

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The effect of trichostatin A (TSA)-induced histone acetylation on the interphase chromatin structure was visualized in vivo with a HeLa cell line stably expressing histone H2A, which was fused to enhanced yellow fluorescent protein. The globally increased histone acetylation caused a reversible decondensation of dense chromatin regions and led to a more homogeneous distribution. These structural changes were quantified by image correlation spectroscopy and by spatially resolved scaling analysis. The image analysis revealed that a chromatin reorganization on a length scale from 200 nm to >1 μm was induced consistent with the opening of condensed chromatin domains containing several Mb of DNA. The observed conformation changes could be assigned to the folding of chromatin during G1 phase by characterizing the effect of TSA on cell cycle progression and developing a protocol that allowed the identification of G1 phase cells on microscope coverslips. An analysis by flow cytometry showed that the addition of TSA led to a significant arrest of cells in S phase and induced apoptosis. The concentration dependence of both processes was studied.

Item Type:Article
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Fejes Tóth, Katalin0000-0001-6558-2636
Additional Information:© 2004 The Company of Biologists Limited. Accepted 26 April 2004. The support of Peter Lichter is gratefully acknowledged. We would like to thank Stephanie Fesser, Sabine Görisch, Karsten Richter, Eberhard Spiess, Andreas Ladurner, Quentin Hanley and Tom Jovin for their help, and Waldemar Waldeck and Jan Ellenberg for valuable discussions. The project was supported by the Volkswagen Foundation in the program 'Junior Research Groups at German Universities'.
Funding AgencyGrant Number
Volkswagen FoundationUNSPECIFIED
Subject Keywords:TSA; Histone deacetylase; Image correlation spectroscopy; Fractal dimension; Cell cycle
Issue or Number:18
Record Number:CaltechAUTHORS:20200421-091446459
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Official Citation:Trichostatin A-induced histone acetylation causes decondensation of interphase chromatin. Katalin Fejes Tóth, Tobias A. Knoch, Malte Wachsmuth, Monika Frank-Stöhr, Michael Stöhr, Christian P. Bacher, Gabriele Müller, Karsten Rippe. Journal of Cell Science 2004 117: 4277-4287; doi: 10.1242/jcs.01293
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102692
Deposited By: Tony Diaz
Deposited On:21 Apr 2020 16:22
Last Modified:21 Apr 2020 16:22

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