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Drosophila caspases involved in developmentally regulated programmed cell death of peptidergic neurons during early metamorphosis

Lee, Gyunghee and Wang, Zixing and Sehgal, Ritika and Chen, Chun-Hong and Kikuno, Keiko and Hay, Bruce and Park, Jae H. (2011) Drosophila caspases involved in developmentally regulated programmed cell death of peptidergic neurons during early metamorphosis. Journal of Comparative Neurology, 519 (1). pp. 34-48. ISSN 0021-9967. doi:10.1002/cne.22498. https://resolver.caltech.edu/CaltechAUTHORS:20200421-151245556

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Abstract

A great number of obsolete larval neurons in the Drosophila central nervous system are eliminated by developmentally programmed cell death (PCD) during early metamorphosis. To elucidate the mechanisms of neuronal PCD occurring during this period, we undertook genetic dissection of seven currently known Drosophila caspases in the PCD of a group of interneurons (vCrz) that produce corazonin (Crz) neuropeptide in the ventral nerve cord. The molecular death program in the vCrz neurons initiates within 1 hour after pupariation, as demonstrated by the cytological signs of cell death and caspase activation. PCD was significantly suppressed in dronc‐null mutants, but not in null mutants of either dredd or strica. A double mutation lacking both dronc and strica impaired PCD phenotype more severely than did a dronc mutation alone, but comparably to a triple dredd/strica/dronc mutation, indicating that dronc is a main initiator caspase, while strica plays a minor role that overlaps with dronc's. As for effector caspases, vCrz PCD requires both ice and dcp‐1 functions, as they work cooperatively for a timely removal of the vCrz neurons. Interestingly, the activation of the Ice and Dcp‐1 is not solely dependent on Dronc and Strica, implying an alternative pathway to activate the effectors. Two remaining effector caspase genes, decay and damm, found no apparent functions in the neuronal PCD, at least during early metamorphosis. Overall, our work revealed that vCrz PCD utilizes dronc, strica, dcp‐1, and ice wherein the activation of Ice and Dcp‐1 requires a novel pathway in addition to the initiator caspases.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/cne.22498DOIArticle
Additional Information:© 2010 Wiley‐Liss, Inc. Funding Information: National Science Foundation. Grant Number: IOS‐0919797. National Institutes of Health. Grant Number: GM070956.
Funders:
Funding AgencyGrant Number
NSFIOS‐0919797
NIHGM070956
Subject Keywords:corazonin; neuropeptide; peptidergic neurons; Drosophila melanogaster; metamorphosis; apoptosis; caspase
Issue or Number:1
DOI:10.1002/cne.22498
Record Number:CaltechAUTHORS:20200421-151245556
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200421-151245556
Official Citation:Lee, G., Wang, Z., Sehgal, R., Chen, C.‐H., Kikuno, K., Hay, B. and Park, J.H. (2011), Drosophila caspases involved in developmentally regulated programmed cell death of peptidergic neurons during early metamorphosis. J. Comp. Neurol., 519: 34-48. doi:10.1002/cne.22498
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102705
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:21 Apr 2020 22:30
Last Modified:16 Nov 2021 18:14

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