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Release and targeting of polycystin-2-carrying ciliary extracellular vesicles

Wang, Juan and Nikonorova, Inna A. and Gu, Amanda and Sternberg, Paul W. and Barr, Maureen M. (2020) Release and targeting of polycystin-2-carrying ciliary extracellular vesicles. Current Biology, 30 (13). R755-R756. ISSN 0960-9822. PMCID PMC7668157. https://resolver.caltech.edu/CaltechAUTHORS:20200422-121506214

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Abstract

Extracellular vesicles (EVs) are emerging as a universal means of cell-to-cell communication and hold great potential in diagnostics and regenerative therapies [1]. An urgent need in the field is a fundamental understanding of physiological mechanisms driving EV generation and function. Ciliary EVs act as signaling devices in Chlamydomonas and Caenorhabditis elegans [2, 3, 4]. Mammalian cilia shed EVs to eliminate unwanted receptors [5] or to retract cilia before entering the cell cycle [6]. Here, we used our established C. elegans model to study sensory-evoked ciliary EV release and targeting using a fluorescently labeled EV cargo polycystin-2 (PKD-2). In C. elegans and mammals, the autosomal dominant polycystic kidney disease (ADPKD) gene products polycystin-1 and polycystin-2 localize to cilia and EVs, act in the same genetic pathway, and function in a sensory capacity, suggesting ancient conservation [7]. We find that males deposit PKD-2-carrying EVs onto the vulva of the hermaphrodite during mating. We also show that mechanical stimulation triggers release of PKD-2-carrying EVs from cilia. To our knowledge, this is the first report of mechanoresponsive ciliary EV release and of the directional transfer of ciliary EVs from one animal to another animal. Since the polycystins are evolutionarily conserved ciliary EV cargoes, our findings suggest that similar mechanisms for EV release and targeting may occur in other systems and biological contexts.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.cub.2020.05.079DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7668157PubMed CentralArticle
https://doi.org/10.1101/2020.04.21.050690DOIDiscussion Paper
ORCID:
AuthorORCID
Sternberg, Paul W.0000-0002-7699-0173
Barr, Maureen M.0000-0003-4483-2952
Alternate Title:Sensory-evoked extracellular vesicle release and targeting, Polycystin-2 ciliary extracellular vesicle release and targeting
Additional Information:© 2020 Elsevier Inc. Available online 6 July 2020.
Funders:
Funding AgencyGrant Number
NIHP30 DK106912
NIHDK059418
NIHDK116606
NIHP40OD010440
Issue or Number:13
PubMed Central ID:PMC7668157
Record Number:CaltechAUTHORS:20200422-121506214
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200422-121506214
Official Citation:Juan Wang, Inna A. Nikonorova, Amanda Gu, Paul W. Sternberg, Maureen M. Barr, Release and targeting of polycystin-2-carrying ciliary extracellular vesicles, Current Biology, Volume 30, Issue 13, 2020, Pages R755-R756, ISSN 0960-9822, https://doi.org/10.1016/j.cub.2020.05.079. (http://www.sciencedirect.com/science/article/pii/S0960982220307661)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102718
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:22 Apr 2020 19:42
Last Modified:18 Nov 2020 18:47

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