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Complementation of V(D)J Recombination Deficiency in RAG-1^(−/−) B Cells Reveals a Requirement for Novel Elements in the N-Terminus of RAG-1

Roman, Christopher A. J. and Cherry, Sara R. and Baltimore, David (1997) Complementation of V(D)J Recombination Deficiency in RAG-1^(−/−) B Cells Reveals a Requirement for Novel Elements in the N-Terminus of RAG-1. Immunity, 7 (1). pp. 13-24. ISSN 1074-7613. https://resolver.caltech.edu/CaltechAUTHORS:20200422-150958808

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Abstract

RAG-1 is an essential component of the site-specific V(D)J recombinase. A new assay system has revealed a significant contribution of the catalytically dispensible N-terminal region of RAG-1 to recombination activity. The foundation for this system is an Abelson virus-transformed cell line derived from RAG-1^(-/-) mice that is dependent on the introduction of exogenous RAG-1 for rearrangement of either plasmid substrates or the endogenous immunoglobulin loci. Use of this line demonstrates that conserved and novel cysteine-containing elements in the N-terminal region are required for full RAG-1 activity when recombination activity is in a RAG-1 dose-responsive range. Our data suggest that the RAG-1 N-terminus enhances the formation of an active recombination complex that facilitates the rearrangement process.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/s1074-7613(00)80506-3DOIArticle
ORCID:
AuthorORCID
Baltimore, David0000-0001-8723-8190
Additional Information:© 1997 Cell Press. Under an Elsevier user license. Received 12 March 1997, Revised 14 May 1997. We thank Ben Chen and Drs. Anthony Koleske and Joel Pomerantz for comments on the manuscript; Ben Chen and Drs. Konstantina Alexandropoulos, Joshy Jacob, Ilana Stancovsky, Kalle Saksela, and Martin Scott for advice and discussions. We are grateful to Drs. Catherine Willett and Lisa Steiner for the zebrafish RAG genomic fragment and RAG-1 sequence prior to publication; Dr. Eugenia Spanopoulou for RAG-1 BR mutants; and Pam Svec for excellent technical assistance. C. A. J. R. was supported by a postdoctoral award from the American Cancer Society. S. R. C. was supported by National Institutes of Health (NIH) predoctoral training grant 5-T32-GM07287. D. B. is an American Cancer Society Scholar. Supported by NIH grant CA51462.
Funders:
Funding AgencyGrant Number
American Cancer SocietyUNSPECIFIED
NIH Predoctoral Fellowship5-T32-GM07287
NIHCA51462
Issue or Number:1
Record Number:CaltechAUTHORS:20200422-150958808
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200422-150958808
Official Citation:Christopher A.J Roman, Sara R Cherry, David Baltimore, Complementation of V(D)J Recombination Deficiency in RAG-1−/− B Cells Reveals a Requirement for Novel Elements in the N-Terminus of RAG-1, Immunity, Volume 7, Issue 1, 1997, Pages 13-24, ISSN 1074-7613, https://doi.org/10.1016/S1074-7613(00)80506-3.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102728
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 Apr 2020 17:04
Last Modified:23 Apr 2020 17:04

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