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Identification of the Abl- and rasGAP-Associated 62 kDa Protein as a Docking Protein, Dok

Yamanashi, Yuji and Baltimore, David (1997) Identification of the Abl- and rasGAP-Associated 62 kDa Protein as a Docking Protein, Dok. Cell, 88 (2). pp. 205-211. ISSN 0092-8674. doi:10.1016/s0092-8674(00)81841-3.

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A 62 kDa protein is highly phosphorylated in many cells containing activated tyrosine kinases. This protein, characterized mainly by its avid association with rasGAP, has proved elusive. Anti-phosphotyrosine antibody was used to purify p62. From peptide sequence, molecular cloning revealed a cDNA encoding a novel protein, p62^(dok), with little homology to others but with a prominent set of tyrosines and nearby sequences suggestive of SH2 binding sites. In cells, v-Abl tyrosine kinase binds and strongly phosphorylates p62^(dok), which then binds rasGAP. A monoclonal antibody, 2C4, to the rasGAP-associated p62 reacts with p62^(dok). Thus, p62^(dok) appears to be the long-sought major substrate of many tyrosine kinases.

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Baltimore, David0000-0001-8723-8190
Additional Information:© 1997 Cell Press. Under an Elsevier user license. Received 24 October 1996, Revised 25 November 1996. Correspondence should be addressed to D. B. We thank R. Cook for micropeptide sequence and valuable discussions. We also thank N. Carpino, D. Wisniewski, A. Strife, D. Marshak, R. Kobayashi, B. Stillman, and B. Clarkson for sharing their results before publication. We are grateful to P. Svec for nucleotide sequence, C. Roman for 1–2 cells, R. Roth for the 2C4 antibody, G. Cohen and N. Shworak for thoughtful suggestions, B. Chen and K. Alexandropoulos for discussions, and T. Koleske and Z. Songyang for critical reading of this manuscript. Y. Y. was a fellow of the Japan–US Cancer Research Training Program. D. B. is an American Cancer Society Research Professor. This work was supported by National Institutes of Health grant CA-51462 (D. B.).
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Japan–US Cancer Research Training ProgramUNSPECIFIED
American Cancer SocietyUNSPECIFIED
Issue or Number:2
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Official Citation:Yuji Yamanashi, David Baltimore, Identification of the Abl- and rasGAP-Associated 62 kDa Protein as a Docking Protein, Dok, Cell, Volume 88, Issue 2, 1997, Pages 205-211, ISSN 0092-8674,
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102736
Deposited By: George Porter
Deposited On:23 Apr 2020 14:58
Last Modified:16 Nov 2021 18:14

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