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Monoclonal Antibodies against the Drosophila Nervous System

Fujita, Shinobu C. and Zipursky, Stephen L. and Benzer, Seymour and Ferrús, Alberto and Shotwell, Sandra L. (1982) Monoclonal Antibodies against the Drosophila Nervous System. Proceedings of the National Academy of Sciences of the United States of America, 79 (24). pp. 7929-7933. ISSN 0027-8424. PMCID PMC347463. doi:10.1073/pnas.79.24.7929.

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A panel of 148 monoclonal antibodies directed against Drosophila neural antigens has been prepared by using mice immunized with homogenates of Drosophila tissue. Antibodies were screened immunohistochemically on cryostat sections of fly heads. A large diversity of staining patterns was observed. Some antigens were broadly distributed among tissues; others were highly specific to nerve fibers, neuropil, muscle, the tracheal system, cell nuclei, photoreceptors, or other structures. The antigens for many of the antibodies have been identified on immunoblots. Monoclonal antibodies that identify specific molecules within the nervous system should prove useful in the study of the molecular genetics of neural development.

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Additional Information:© 1982 by the National Academy of Sciences. Contributed by Seymour Benzer, September 28, 1982. We thank Marika Szalay and Devra Spurr for skillful technical assistance, David Levy for introduction to hybridoma techniques, and Jeremy Brockes, Katherine Stygall, David Balzer, Gregg Lemke, Mark Tanouye, Obaid Siddiqi, Tadmiri Venkatesh, David Teplow, and Lawrence Kauvar for help and discussion. This work was supported by Grant PCM 79-11771 from the National Science Foundation and by fellowships from the Gosney (A.F.), Lawrence E. Hanson (S.L.S.), and Helen Hay Whitney (S.L.Z.) Foundations. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
NXSFPCM 79-11771
Gosney FoundationUNSPECIFIED
Lawrence E. Hanson FoundationUNSPECIFIED
Helen Hay Whitney FoundationUNSPECIFIED
Subject Keywords:neuroimmunology; immunohistochemistry; brain; retina; immunoblots
Issue or Number:24
PubMed Central ID:PMC347463
Record Number:CaltechAUTHORS:FUJpnas82
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Official Citation:Monoclonal antibodies against the Drosophila nervous system S C Fujita, S L Zipursky, S Benzer, A Ferrús, S L Shotwell Proceedings of the National Academy of Sciences Dec 1982, 79 (24) 7929-7933; DOI: 10.1073/pnas.79.24.7929
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10275
Deposited By: Archive Administrator
Deposited On:21 Apr 2008
Last Modified:08 Nov 2021 21:06

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