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HIV-1 Messenger RNA in Peripheral Blood Mononuclear Cells as an Early Marker of Risk for Progression to AIDS

Saksela, Kalle and Stevens, Cladd E. and Rubinstein, Pablo and Taylor, Patricia E. and Baltimore, David (1995) HIV-1 Messenger RNA in Peripheral Blood Mononuclear Cells as an Early Marker of Risk for Progression to AIDS. Annals of Internal Medicine, 123 (9). pp. 641-648. ISSN 0003-4819. doi:10.7326/0003-4819-123-9-199511010-00001.

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Objective: To establish human immunodeficiency virus type 1 (HIV-1) messenger RNA (mRNA) expression in peripheral blood mononuclear cells as a marker of risk for progression to the acquired immunodeficiency syndrome (AIDS) in a large cohort of HIV-infected persons followed for a prolonged period. Design: Retrospective testing of cryopreserved, coded specimens. Setting: Research laboratories at the New York Blood Center and the Rockefeller University. Patients: 150 homosexual men infected with HIV-1 who did not have an AIDS diagnosis at the time of testing. Measurements: Multiply spliced and unspliced HIV-1 mRNAs in total peripheral blood mononuclear cell RNA were quantitated using reverse transcriptase-initiated polymerase chain reaction (PCR) and compared with other laboratory data and clinical outcome during the subsequent 8 years. Results: Although HIV-1 mRNA expression generally correlated with immunologic status, it was associated with future disease progression independently of CD4+ cell counts or their rate of decrease at the time of sampling. The association of HIV-1 mRNA with disease progression in persons with CD4+ cell counts higher than the median (> 624 cells/mm³) was particularly noteworthy; further variation in the CD4+ cell counts within this group was not prognostically significant. Conclusions: The expression of HIV-1 mRNA in peripheral blood mononuclear cells is a strong independent marker for future HIV disease progression, even in persons with normal T-cell subsets.

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Baltimore, David0000-0001-8723-8190
Additional Information:© 1995 American College of Physicians.
Issue or Number:9
Record Number:CaltechAUTHORS:20200424-091648599
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102768
Deposited By: Tony Diaz
Deposited On:24 Apr 2020 17:27
Last Modified:16 Nov 2021 18:15

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