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Functional characterization of dopaminergic neurons derived from rodent mesencephalic progenitor cells

Storch, Alexander and Lester, Henry A. and Boehm, Bernhard O. and Schwarz, Johannes (2003) Functional characterization of dopaminergic neurons derived from rodent mesencephalic progenitor cells. Journal of Chemical Neuroanatomy, 26 (2). pp. 133-142. ISSN 0891-0618. https://resolver.caltech.edu/CaltechAUTHORS:20200427-151732404

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Abstract

Neural progenitor cells existing in the developing and adult brain retain the capacity to self renew and to produce the major cell types of the brain opening new avenues for restorative therapy of neuropsychiatric disorders. These cells can be grown in vitro while retaining the potential to differentiate into nervous tissue. A primary target for neurorestoration is Parkinson's disease, characterized by a continuous loss of the dopaminergic neurons in the substantia nigra pars compacta leading to dopamine depletion in the striatum and subsequent clinical symptoms including bradykinesia, rigidity and tremor. We established a protocol for long-term expansion and dopaminergic differentiation of rodent and human mesencephalic neural progenitor cells. Here we perform functional studies using both biochemical and electrophysiological techniques on dopaminergic neurons derived from rodent mesencephalic progenitor cells labeled with tyrosine hydroxylase (TH) gene promotor-driven expression of enhanced green fluorescence protein (EGFP). Thus, we demonstrate that these cells produce and release dopamine, express voltage-gated potassium and sodium currents, and fire action potentials. Furthermore, we detect a slowly activating hyperpolarization-activated inward cation current (Ih), which is specific for dopaminergic neurons among present midbrain neurons. Our results demonstrate that differentiated mesencephalic progenitors exhibit some major morphological and functional characteristics of dopaminergic neurons. Therefore, these neural progenitor cells might serve as a useful source of dopaminergic neurons for studying the development and degeneration of these cells and may further serve as a continuous, on-demand source of cells for therapeutic transplantation in Parkinson's disease.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/s0891-0618(03)00067-xDOIArticle
ORCID:
AuthorORCID
Lester, Henry A.0000-0002-5470-5255
Additional Information:© 2003 Elsevier B.V. Received 6 September 2002, Revised 1 April 2003, Accepted 28 June 2003, Available online 26 September 2003.
Subject Keywords:Parkinson's disease; Neuroregeneration; Dopaminergic neurons; Transplantation; Neural progenitor cell; Patch–clamp recordings
Issue or Number:2
Record Number:CaltechAUTHORS:20200427-151732404
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200427-151732404
Official Citation:Alexander Storch, Henry A. Lester, Bernhard O. Boehm, Johannes Schwarz, Functional characterization of dopaminergic neurons derived from rodent mesencephalic progenitor cells, Journal of Chemical Neuroanatomy, Volume 26, Issue 2, 2003, Pages 133-142, ISSN 0891-0618, https://doi.org/10.1016/S0891-0618(03)00067-X. (http://www.sciencedirect.com/science/article/pii/S089106180300067X)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102819
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:27 Apr 2020 22:24
Last Modified:27 Apr 2020 22:24

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