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Multiple Nuclear Factors Interact with the Immunoglobulin Enhancer Sequences

Sen, Ranjan and Baltimore, David (2006) Multiple Nuclear Factors Interact with the Immunoglobulin Enhancer Sequences. Journal of Immunology, 177 (11). pp. 7485-7496. ISSN 0022-1767. https://resolver.caltech.edu/CaltechAUTHORS:20200428-073832282

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Abstract

To characterize proteins that bind to the immunoglobulin (Ig) heavy chain and the κ light chain enhancers, an electrophoretic mobility shift assay with end-labeled DNA fragments was used. Three binding proteins have been found. One is NF-A, a factor found in all tested cell types that binds to the octamer sequence found upstream of all Ig variable region gene segments and to the same octamer in the heavy chain enhancer. The second, also ubiquitous, protein binds to a sequence in both the heavy chain and the κ enhancers that was previously shown to be protected from methylation in vivo. Other closely related sites do not compete for this binding, implying a restriction enzyme-like binding specificity. The third protein binds to a sequence in the κ enhancer (and to an identical sequence in the SV40 enhancer) and is restricted in its occurrence to B cells.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://www.jimmunol.org/content/177/11/7485PublisherArticle
https://doi.org/10.1016/0092-8674(86)90346-6DOIArticle from Cell
ORCID:
AuthorORCID
Baltimore, David0000-0001-8723-8190
Additional Information:© 1986 by Cell Press. Reprinted from Cell 46: 705–716, © 1986, with permission from Elsevier. Received March 19, 1986; revised June 5, 1986. We would like to thank David Weaver, Lou Staudt, and Nancy Speck for their help in generating the "library" of extracts described, Judah Weinberger and Philip Sharp for communicating their results prior to publication, and Ginger Pierce for quick and efficient typing of the manuscript. R. S. was a recipient of a fellowship from the Damon Runyon-Walter Winchell Cancer Fund. This work was supported by a grant from the American Cancer Society awarded to D. B. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
Damon Runyon-Walter Winchell Cancer FundUNSPECIFIED
American Cancer SocietyUNSPECIFIED
Issue or Number:11
Record Number:CaltechAUTHORS:20200428-073832282
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200428-073832282
Official Citation:Pillars Article: Multiple Nuclear Factors Interact with the Immunoglobulin Enhancer Sequences. Cell 1986. 46: 705–716. Ranjan Sen, David Baltimore. The Journal of Immunology December 1, 2006, 177 (11) 7485-7496;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102827
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:28 Apr 2020 15:48
Last Modified:28 Apr 2020 15:48

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