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The Drosophila MicroRNA Mir-14 Suppresses Cell Death and Is Required for Normal Fat Metabolism

Xu, Peizhang and Vernooy, Stephanie Y. and Guo, Ming and Hay, Bruce A. (2003) The Drosophila MicroRNA Mir-14 Suppresses Cell Death and Is Required for Normal Fat Metabolism. Current Biology, 13 (9). pp. 790-795. ISSN 0960-9822. doi:10.1016/s0960-9822(03)00250-1.

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MicroRNAs (miRNAs) are small regulatory RNAs that are between 21 and 25 nucleotides in length and repress gene function through interactions with target mRNAs 1, 2. The genomes of metazoans encode on the order of several hundred miRNAs [3], but the processes they regulate have been defined for only two in C. elegans4, 5. We searched for new inhibitors of apoptotic cell death by testing existing collections of P element insertion lines for their ability to enhance a small-eye phenotype associated with eye-specific expression of the Drosophila cell death activator Reaper. Here we report the identification of the Drosophila miRNA mir-14 as a cell death suppressor. Loss of mir-14 enhances Reaper-dependent cell death, whereas ectopic expression suppresses cell death induced by multiple stimuli. Animals lacking mir-14 are viable. However, they are stress sensitive and have a reduced lifespan. Mir-14 mutants have elevated levels of the apoptotic effector caspase Drice, suggesting one potential site of action. Mir-14 also regulates fat metabolism. Deletion of mir-14 results in animals with increased levels of triacylglycerol and diacylglycerol, whereas increases in mir-14 copy number have the converse effect. We discuss possible relationships between these phenotypes.

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Additional Information:© 2003 Cell Press. Published by Elsevier Ltd. Received 14 March 2003, Revised 28 March 2003, Accepted 28 March 2003, Available online 1 May 2003. We thank Hong Yu for invaluable technical support, Rosalind Young for help with histology, Pankaj Kapahi for valuable discussions on fly aging and fat metabolism, David Mathog for help with the analysis of miRNA binding sites, and Paul Fisher for the gift of anti-lamin antibody. This work was supported by a Gosney fellowship to P.X. and grants to B.A.H. from the National Institutes of Health (GM057422) and The Ellison Medical Foundation.
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Gosney FoundationUNSPECIFIED
Ellison Medical FoundationUNSPECIFIED
Issue or Number:9
Record Number:CaltechAUTHORS:20200428-080415962
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Official Citation:Peizhang Xu, Stephanie Y. Vernooy, Ming Guo, Bruce A. Hay, The Drosophila MicroRNA Mir-14 Suppresses Cell Death and Is Required for Normal Fat Metabolism, Current Biology, Volume 13, Issue 9, 2003, Pages 790-795, ISSN 0960-9822, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102829
Deposited By: Tony Diaz
Deposited On:28 Apr 2020 15:38
Last Modified:16 Nov 2021 18:15

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