CaltechAUTHORS
  A Caltech Library Service

A Fluctuation Method to Quantify In Vivo Fluorescence Data

Rosenfeld, Nitzan and Perkins, Theodore J. and Alon, Uri and Elowitz, Michael B. and Swain, Peter S. (2006) A Fluctuation Method to Quantify In Vivo Fluorescence Data. Biophysical Journal, 91 (2). pp. 759-766. ISSN 0006-3495. PMCID PMC1483091. https://resolver.caltech.edu/CaltechAUTHORS:20200428-123053062

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20200428-123053062

Abstract

Quantitative in vivo measurements are essential for developing a predictive understanding of cellular behavior. Here we present a technique that converts observed fluorescence intensities into numbers of molecules. By transiently expressing a fluorescently tagged protein and then following its dilution during growth and division, we observe asymmetric partitioning of fluorescence between daughter cells at each division. Such partition asymmetries are set by the actual numbers of proteins present, and thus provide a means to quantify fluorescence levels. We present a Bayesian algorithm that infers from such data both the fluorescence conversion factor and an estimate of the measurement error. Our algorithm works for arbitrarily sized data sets and handles consistently any missing measurements. We verify the algorithm with extensive simulation and demonstrate its application to experimental data from Escherichia coli. Our technique should provide a quantitative internal calibration to systems biology studies of both synthetic and endogenous cellular networks.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1529/biophysj.105.073098DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1483091PubMed CentralArticle
ORCID:
AuthorORCID
Elowitz, Michael B.0000-0002-1221-0967
Additional Information:© 2006 The Biophysical Society. Published by Elsevier Inc. Received 22 August 2005, Accepted 19 April 2006, Available online 6 January 2009. We thank Derek Bowie, Robert Sidney Cox III, Jon Young, and the anonymous referees for useful comments. M.B.E. acknowledges a CASI award from the Burroughs Wellcome Fund and the Searle Scholars Program. U.A. and M.B.E. are supported by the Human Frontiers Science Program. P.S.S. is supported by the National Sciences and Engineering Research Council (Canada) and by a Tier II Canada Research Chair.
Funders:
Funding AgencyGrant Number
Burroughs Wellcome FundUNSPECIFIED
Searle Scholars ProgramUNSPECIFIED
Human Frontier Science ProgramUNSPECIFIED
Natural Sciences and Engineering Research Council of Canada (NSERC)UNSPECIFIED
Canada Research Chairs ProgramUNSPECIFIED
Issue or Number:2
PubMed Central ID:PMC1483091
Record Number:CaltechAUTHORS:20200428-123053062
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200428-123053062
Official Citation:Nitzan Rosenfeld, Theodore J. Perkins, Uri Alon, Michael B. Elowitz, Peter S. Swain, A Fluctuation Method to Quantify In Vivo Fluorescence Data, Biophysical Journal, Volume 91, Issue 2, 2006, Pages 759-766, ISSN 0006-3495, https://doi.org/10.1529/biophysj.105.073098. (http://www.sciencedirect.com/science/article/pii/S000634950671774X)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102877
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:28 Apr 2020 19:38
Last Modified:28 Apr 2020 19:38

Repository Staff Only: item control page