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Monitoring Activity of Caspases and Their Regulators in Yeast Saccharomyces cerevisiae

Hawkins, Christine J. and Wang, Susan L. and Hay, Bruce A. (2000) Monitoring Activity of Caspases and Their Regulators in Yeast Saccharomyces cerevisiae. In: Apoptosis. Methods in Enzymology. No.322. Academic Press , London, pp. 162-174. ISBN 9780121822231.

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Caspases are a family of site-specific proteases that play important signaling and effector roles in most apoptotic death pathways. Caspases are made as zymogens and become activated after cleavage. Caspase activity is regulated by both activators and inhibitors. Yeast provide a useful system in which to characterize the function of caspases and their regulators because yeast appear to lack endogenous caspases as well as other components of the apoptotic machinery. Also, yeast are genetically the most tractable eukaryote with which to work. Finally, transformation occurs at a high frequency, making them suitable for library screening or large-scale mutagenesis. In addition to caspases, yeast systems have also been used to analyze other cell death pathway components.2, 3, 4, 5, 6, 7, 8, 9, 10, 11 These approaches are discussed in [27] and [28].

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Additional Information:© 2000 Academic Press. Published by Elsevier Inc. C.J.H. was supported by a fellowship from the Human Frontiers Science Program. B.A.H. is a Searle Scholar. This work was supported by grants to B.A.H. from the National Institutes of Health (GM057422-01), the Ellison Medical Foundation and the Burroughs Wellcome Fund (New Investigator Award in the Pharmacological Sciences).
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Human Frontier Science ProgramUNSPECIFIED
Searle Scholars ProgramUNSPECIFIED
Ellison Medical FoundationUNSPECIFIED
Burroughs Wellcome FundUNSPECIFIED
Series Name:Methods in Enzymology
Issue or Number:322
Record Number:CaltechAUTHORS:20200428-142551477
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Official Citation:Christine J. Hawkins, Susan L. Wang, Bruce A. Hay, [14] - Monitoring Activity of Caspases and Their Regulators in Yeast Saccharomyces cerevisiae, Editor(s): John C. Reed, Methods in Enzymology, Academic Press, Volume 322, 2000, Pages 162-174, ISSN 0076-6879, ISBN 9780121822231, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102887
Deposited By: Tony Diaz
Deposited On:28 Apr 2020 21:55
Last Modified:16 Nov 2021 18:16

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