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P element insertion-dependent gene activation in the Drosophila eye

Hay, Bruce A. and Maile, Randy and Rubin, Gerald M. (1997) P element insertion-dependent gene activation in the Drosophila eye. Proceedings of the National Academy of Sciences of the United States of America, 94 (10). pp. 5195-5200. ISSN 0027-8424. PMCID PMC24655. https://resolver.caltech.edu/CaltechAUTHORS:20200428-153907305

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Abstract

Insights into the function of a gene can be gained in multiple ways, including loss-of-function phenotype, sequence similarity, expression pattern, and by the consequences of its misexpression. Analysis of the phenotypes produced by expression of a gene at an abnormal time, place, or level may provide clues to a gene’s function when other approaches are not illuminating. Here we report that an eye-specific, enhancer–promoter present in the P element expression vector pGMR is able to drive high level expression in the eye of genes near the site of P element insertion. Cell fate determination, differentiation, proliferation, and death are essential for normal eye development. Thus the ability to carry out eye-specific misexpression of a significant fraction of genes in the genome, given the dispensability of the eye for viability and fertility of the adult, should provide a powerful approach for identifying regulators of these processes. To test this idea we carried out two overexpression screens for genes that function to regulate cell death. We screened for insertion-dependent dominant phenotypes in a wild-type background, and for dominant modifiers of a reaper overexpression-induced small eye phenotype. Multiple chromosomal loci were identified, including an insertion 5′ to hid, a potent inducer of apoptosis, and insertions 5′ to DIAP1, a cell death suppressor. To facilitate the cloning of genes near the P element insertion new misexpression vectors were created. A screen with one of these vectors identified eagle as a suppressor of a rough eye phenotype associated with overexpression of an activated Ras1 gene.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1073/pnas.94.10.5195DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC24655PubMed CentralArticle
Additional Information:© 1997 The National Academy of Sciences of the USA. Contributed by Gerald M. Rubin, February 19, 1997. We thank Todd Laverty for chromosome in situ hybridizations, Paula Sicurello for scanning electron microscopy; Chris Suh for sequencing; Kristen White (Massachusetts General Hospital) for providing some initial mapping data that placed the GMR-HAFAS110 insertion near hid; Henry Chang for preparing the figures; and Alan C. Spradling, Pernille Rorth, and Ilaria Rebay for helpful comments on the manuscript. This work was supported by the Howard Hughes Medical Institute (G.M.R.), and by an American Cancer Society, California Division, Senior Postdoctoral Fellowship (B.A.H.). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
American Cancer Society, California DivisionUNSPECIFIED
Issue or Number:10
PubMed Central ID:PMC24655
Record Number:CaltechAUTHORS:20200428-153907305
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200428-153907305
Official Citation:P element insertion-dependent gene activation in the Drosophila eye. Bruce A. Hay, Randy Maile, Gerald M. Rubin. Proceedings of the National Academy of Sciences May 1997, 94 (10) 5195-5200; DOI: 10.1073/pnas.94.10.5195
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102894
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:28 Apr 2020 22:56
Last Modified:28 Apr 2020 22:56

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