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Differential requirements for the Pax6(5a) genes eyegone and twin of eyegone during eye development in Drosophila

Yao, Jih-Guang and Weasner, Bonnie M. and Wang, Lan-Hsin and Jang, Chuen-Chuen and Weasner, Brandon and Tang, Chiou-Yang and Salzer, Claire L. and Chen, Chun-Hong and Hay, Bruce and Sun, Y. Henry and Kumar, Justin P. (2008) Differential requirements for the Pax6(5a) genes eyegone and twin of eyegone during eye development in Drosophila. Developmental Biology, 315 (2). pp. 535-551. ISSN 0012-1606. PMCID PMC2702218. https://resolver.caltech.edu/CaltechAUTHORS:20200429-085102107

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Abstract

In eye development the tasks of tissue specification and cell proliferation are regulated, in part, by the Pax6 and Pax6(5a) proteins respectively. In vertebrates, Pax6(5a) is generated as an alternately spliced isoform of Pax6. This stands in contrast to the fruit fly, Drosophila melanogaster, which has two Pax6(5a) homologs that are encoded by the eyegone and twin of eyegone genes. In this report we set out to determine the respective contributions that each gene makes to the development of the fly retina. Here we demonstrate that both eyg and toe encode transcriptional repressors, are expressed in identical patterns but at significantly different levels. We further show, through a molecular dissection of both proteins, that Eyg makes differential use of several domains when compared to Toe and that the number of repressor domains also differs between the two Pax6(5a) homologs. We predict that these results will have implications for elucidating the functional differences between closely related members of other Pax subclasses.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.ydbio.2007.12.037DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2702218PubMed CentralArticle
Additional Information:© 2008 Elsevier Inc. Received 29 August 2007, Revised 29 November 2007, Accepted 11 December 2007, Available online 4 January 2008. We thank Cheng-ting Chien, Gwo-Jen Liaw, Peter Cherbas and Thom Kaufman for valuable suggestions. We thank Chun-Lan Hsu and Yu-Chi Yang for preparing fly food and maintaining fly stocks. This work was supported by grants to Y.H.S from the National Science Council (NSC93-2321-B-001-010 and 94-2321-B-001-011) of the Republic of China and a grant from the National Institutes of Health (R01 EY014863) to J.P.K.
Funders:
Funding AgencyGrant Number
National Science Council (Taipei)93-2321-B-001-010
National Science Council (Taipei)94-2321-B-001-011
NIHR01-EY014863
Subject Keywords:Pax6(5a); Eyegone; Twin of eyegone; Retina; Eye; Drosophila
Issue or Number:2
PubMed Central ID:PMC2702218
Record Number:CaltechAUTHORS:20200429-085102107
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20200429-085102107
Official Citation:Jih-Guang Yao, Bonnie M. Weasner, Lan-Hsin Wang, Chuen-Chuen Jang, Brandon Weasner, Chiou-Yang Tang, Claire L. Salzer, Chun-Hong Chen, Bruce Hay, Y. Henry Sun, Justin P. Kumar, Differential requirements for the Pax6(5a) genes eyegone and twin of eyegone during eye development in Drosophila, Developmental Biology, Volume 315, Issue 2, 2008, Pages 535-551, ISSN 0012-1606, https://doi.org/10.1016/j.ydbio.2007.12.037. (http://www.sciencedirect.com/science/article/pii/S0012160607015953)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:102900
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:29 Apr 2020 16:02
Last Modified:29 Apr 2020 16:02

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